目　录

作者简介

工作摄影

家庭田园

风景摄影

序　一

序　二

前　言

第一篇　综　述

高等植物酶合成的调节——基因表达

一、序言

二、Jacod-Monod操纵子学说及其发展

三、真核生物基因表达的特点

1.转录和翻译的地点分离

2.基因组的复杂性

3.DNA与核蛋白有密切的关系

4.基因调节的方式更复杂多样

四、高等植物基因活性的调节

1.细胞内调节

2.细胞间调节——激素

3.外界环境的调节

4.发育分化过程中基因的调节［8，10］

五、结语

参考文献

Reviews Induction and regulation of cellulase synthesis in Trichoderma pseudokoningii mutants EA3-867 and N2-78

Introduction

Materials and methods

Organism

Culture

Induction

Preparation of enzyme extract

Preparation of cellulase for separation and purification

Assay

Chromatography

Electrophoresis

Ultracentrifugation

Results

Comparison of different carbon sources as cellulase inducers

Effect of sophorose，cellobiose and cellulose added to glycerol culture upon cellulase induction

Cellulase components induced by sophorose and cellulose

Cellulase induction by sophorose in washed mycelium

Catabolite repression

Changes in the regulatory mechanism of cellulase synthesis in the mutants

Separation，purification and properties of cellulase components of N2-78

Isolation of PolyA-RNA from mycelium of N2-78 induced by sophorose

Discussion

Acknowledgements

References

Organization and Expression of Genes for Photosynthetic Pigments-Protein Complexes in Photosynthetic Bacteria

Metabolic Versatility

Photosynthetic Apparatus

Organization of Genes Coding for LH，RC，and Pigment Biosynthetic Enzymes and Cytochromes

Regulation of Expression of the Genes Coding for LH，RC，Bchl，and Crt Biosynthesis

Coordination and Interaction of the Genes for LH，RC，Bchl，and Crt Biosynthesis

Environmental Stimuli

Oxygen

Light Intensity

Regulation of the puf Operon

Conclusion

References

The Nicotiana Chloroplast Genome

Isolation and Restriction Analysis

A.Isolation and Purification

B.Restriction Enzyme Analysis

Properties and Organization

Diversity and Evolution

Cloning and Gene Library

Mapping and Expression

A.Mapping of ct-DNA and location of genes for the LS of RuBPCase and 16s，23s and 5s rRNA

B.Expression of ct-DNA in E.coli and B.subtilis

Concluding Remarks

References

国外纤维素酶研究概况

一、序言

二、纤维素酶的诱导和作用

1.纤维素酶的分布

2.胞外酶和胞内酶

3.纤维素酶的诱导形成

4.纤维素酶的分泌和表面活性剂的作用

5.纤维素酶的作用。

三、纤维底物结构与酶作用的关系

1.纤维的结构、成分和微纤维结构的几种假设

2.纤维结构对酶作用敏感性的影响

四、国外提高酶解效率的途径

1.酶活力方面

2.纤维底物方面

五、结束语

参考文献

纤维素酶研究进展

一、纤维素酶的作用机理

1.纤维素酶的组分

2.纤酶素酶的性质及作用方式

3.纤维素酶作用的化学模拟

4.纤维素酶的抑制剂

5.纤维素酶作用的动力学

二、纤维素酶的诱导合成及调节控制

三、纤维素酶的应用

四、结语

参考文献

第二篇　植物生理和代谢调节（1961—1965）

棉铃脱落与碳水化合物化谢的关系

一、引言

二、材料与方法

1.显微组织化学的方法

2.化学分析的测定

三、试验结果

1.脱落过程中离层细胞的淀粉转化规律

2.离层形成与碳水化合物代谢的关系

3.半乳糖对离层形成的影响

四、讨论

结论提要

参考文献

THE RELATION BETWEEN CARBOHYDRATE METABOLISM AND BOLL SHEDDING IN COTTON PLANT

植物呼吸代谢的生理意义Ⅰ.器官脱落与呼吸途径的联系

一、引言

二、材料与方法

三、试验结果

1.各种碳水化合物及有关中间代谢物对离体黄杨叶柄脱落的影响

2.吲哚乙酸（IAA）与抗坏血酸对离体黄杨叶柄脱落的影响

3.不同呼吸途径抑制剂对离体黄杨叶柄脱落的影响

四、讨论

参考文献

THE PHYSIOLOGICAL SIGNIFICANCE OF RESPIRATORY METABOLISM IN PLANTSⅠ.THE RESPIRATORY PATHWAYS IN RELATION TO ABSCISSION

植物的脂肪合成及其调节Ⅰ.油菜种子形成期间葡萄糖降解的途径

一、引言

二、材料与方法

1.材料

2.方法

三、结果与讨论

1.EMP途径的酶系活力测定

2.HMP途径的酶系活力测定

参考文献

FAT SYNTHESIS AND ITS REGULATION IN PLANTSⅠ.ENZYME SYSTEMS OF GLUCOSE DEGRADATION IN RAPE SEEDS DURING RIPENING

植物的脂肪合成及其调节Ⅲ.油菜籽实形成过程中代谢途径的变化与内源抑制剂的调节作用

一、引言

二、试验材料和方法

1.材料

2.方法

三、试验结果

1.油菜籽实形成过程中HMP和EMP途径酶系活力的变化

2.油菜籽实形成过程中呼吸途径的变化

3.油菜内源抑制剂对代谢途径的调节作用

四、讨论

参考文献

FAT SYNTHESIS AND ITS REGULATION IN PLANTSⅢ.THE CHANGES OF METABOLIC PATHWAYS AND THE REGULATORY ACTION OF AN ENDOGENOUS INHIBITOR IN THE DFVELOPING SILIQUES OF BRASSICA NAPUS LINN.

植物的脂肪合成及其调节Ⅵ.油菜种子脂肪合成与HMP途径关系的同位素证据

一、引言

二、材料与方法

1.材料

2.方法

三、试验结果

1.种子形成过程中和比的变化

2.标记葡萄糖转化产物分离试验和HMP途径的定量计算

四、讨论

参考文献

FAT SYNTHESIS AND ITS REGULATION IN PLANTSⅥ.ISOTOPE EVIDENCE FOR THE RELATION OF FAT SYNTHESIS BY RAPE SEEDS TO THE HMP PATHWAY

植物的脂肪合成及其调节Ⅶ.大豆种子形成过程中物质累积与呼吸代谢的关系

一、前言

二、材料和方法

1.材料

2.方法

三、试验结果

1.大豆种子形成过程中糖、总脂、蛋白、可溶氮和磷化合物的变化

2.呼吸测定

3.种子形成过程中两途径酶活力的变化

四、讨论

参考文献

Fat Synthesis and Its Regulation in PlantsⅦ.The Accumulation of Organic Compounds in Soybean Seeds During Seed Development in Relation to The Respiratory Pathways

植物的脂肪合成及其调节Ⅸ.NADPH2的氧化速率对油菜种子HMP途径和脂肪形成的调节作用

一、前言

二、材料与方法

1.材料

2.方法

三、实验结果

1.葡萄糖C-1氧化限制步骤的分析

2.人工电子受体试验

3.天然电子受体试验

四、讨论

参考文献

Fat Synthesis and Its Regulation in PlantsⅨ.The Regulatory Action of the Oxidation Rate of NADPH2 in the HMP Pathway and Fat Synthesis in Rape Seeds

The Changes of Metabolic Pathways and the Regulatory Action of an Endogenous Inhibitor in the Developing Siliques of Brassica Napus Linn

Introduction

Materials and Methods

Experimental Results

1.Changes of the Enzymic Activities of the HMP and the EMP Pathways during the Development of Rape Siliques

2.Changes of Respiratory Pathways during the Deveiopment of Rape Seeds

3.The Regulation of Metabolic Pathways by an Endogenous Inhibitor

Discussion

References

第三篇　微生物发酵工程及纤维素酶在工农医上的应用（1967—1980）

纤维素酶固体曲的制取及其在酒精生产上的试用

一、前言

二、纤曲的制造

1.工艺流程

2.菌种的培养

3.厚层通风曲的培养

4.纤曲的质量检验

三、发酵试验

1.测定方法

2.加纤曲的发酵试验

3.发酵结果的误差分析

四、结果讨论

五、小结

六、存在问题与今年打算

参考文献

二株高活力纤维素分解菌EA3-867和N2-78的获得及其特性的比较

一、材料和方法

1.菌种

2.培养基

3.诱变方法和菌种选育系谱

4.酶制剂制备

5.酶活力测定

6.可溶性蛋白质［14］

二、结果

1.诱变菌株和野生菌株的形态比较

2.变异菌株和野生型菌株的纤维素酶活力比较

3.变异菌株和野生型菌株纤维素酶特性的比较

三、讨论

参考文献

Isolation of Two Mutant Strains of Trichoderma Pseudokoningii Rifai EA3-867 and N2-78 with High Cellulase Yields and Comparison of Their Characteristics

木霉纤维素酶的诱导形成及其调节Ⅰ.槐糖对木霉EA3-867纤维素酶形成的诱导作用

一、材料和方法

1.菌种

2.试剂

3.槐糖的提取、纯化、结晶和鉴定

4.液体培养方法

5.酶活力测定

6.糖、甘油和生长的测定

7.糖的纸上层析

8.酶组分的凝胶电泳分离法

三、结果

1.不同碳源中生长和纤维素酶形成的比较

2.槐糖、纤维二糖和纤维素粉对木霉EA3-867甘油培养物纤维素酶形成的影响

3.槐糖和纤维素粉对不同菌种甘油培养物形成纤维素酶的影响

4.槐糖和纤维素粉诱导的纤维素酶组分的比较

四、讨论

参考文献

Induction and Regulation of Cellulase Formation in TrichodermaⅠ.The Induction of Cellulase Formation in Trichoderma EA3-867 by Sophorose

木霉纤维素酶的诱导形成及其调节Ⅱ.槐糖对木霉EA3-867洗涤菌丝体纤维素酶形成的诱导作用及降解物阻遏现象

一、引言

二、材料和方法

1.菌种

2.试剂

3.方法

三、试验结果

1.洗涤菌丝体纤维素酶的诱导形成

2.降解物阻遏

四、讨论

参考文献

Induction and Regulation of Cellulase Formation in TrichodermaⅡ.Induction of Cellulase by Sophorose in Washed Mycelia of T.Pseudokoningii EA3-867 and its Catabolite Repression

木霉纤维素酶的诱导形成及其调节Ⅲ.葡萄糖母液和槐豆荚提取液对纤维素酶诱导效应的分析

一、引言

二、材料与方法

1.菌种

2.试剂

3.方法

三、试验结果

1.不同浓度葡萄糖母液的诱导作用比较

2.葡萄糖母液中诱导剂的分离和鉴定

3.槐豆荚提取液的诱导作用

四、讨论

参考文献

Induction and Regulation of Cellulase Formation in TrichodermaⅢ.The Inductive Formation of Cellulase by Glucose Waste Molasses and Extracts of Pods of Sophora Japonica L.

木霉纤维素酶的诱导形成及其调节Ⅳ.高产变异株纤维素酶合成调节的变化——酶活提高原因的初步分析

一、材料和方法

二、试验结果

1.变异株和野生型菌株菌落大小和生长的差异

2.变异株和野生型菌株在不同培养基中纤维素酶活性的比较

3.变异株和野生型菌株纤维素酶凝胶电泳的比较

4.变异株和野生型菌株纤维素酶诱导形成时对槐糖诱导和葡萄糖阻遏敏感性的比较

三、讨论

参考文献

Induction and Regulation of Cellulase Forma Tion in Trichoderma.Ⅳ.Changes in Regu Latory Mechanisms of Cellulase Synthesis of Two Mutants with High Cellulase Yields——A Preliminary Analysis of the Mechanism of Increase in Cellulase Production

木霉纤维素酶的诱导形成及其调节Ⅴ.拟康氏木霉（Trichoderma pseudokoningii Rafai）N2-78洗涤菌丝体纤维素酶诱导形成过程中核酸代谢的变化

一、引言

二、材料和方法

1.菌种(https://www.daowen.com)

2.方法

三、试验结果

1.N2-78洗涤菌丝体被槐糖诱导不同时间纤维素酶、蛋白和核酸含量的变化

2.菌丝体总RNA的抽提

3.菌丝体Pol（yA）-RNA的分离

四、讨论

参考文献

Induction and Regulation of Cellulase Formation in Trichoderma V.The Changes in Nucleic Acid Metabolism of Washed Mycelia of Trichoderma Pseudokoningii Rafai N2-78 During Inductive Formation of Cellulase

木霉纤维素酶的诱导形成及其调节Ⅵ.拟康氏木霉（Trichoderma pseudokoningii Rafai）N2-78被槐糖诱导形成的纤维素酶组分的分离、纯化及性质

一、材料与方法

1.菌种

2.试剂

3.方法

二、试验结果

1.纤维素酶组分的分离与纯化

2.C1酶的纯度鉴定

3.纤维素酶纯化酶的性质

三、讨论

参考文献

Induction and Regulation of Cellulase Formation in TrichodermaⅥ.Separation，Purification and Properties of Cellulase Components of Trichoderma Pseudokonirngii Rafai N2-78 Induced by Sophorose

第四篇　光合作用和叶绿体基因表达的光氧调控（1980—1986）

Nicotiana Chloroplast GenomeⅠ.Chloroplast DNA Diversity

Introduction

Materials and Methods

Plant Material

Purification of Chloroplast

Isolation of Chloroplast DNA

Restriction Enzyme Analysis of Chloroplast DNA

Results

Discussion

References

Nicotiana Chloroplast GenomeⅡ.Chloroplast DNA Alteration

Introduction

Materials and Methods

Results and Discussion

Conclusion

Acknowledgement

References

Nicotiana Chloroplast GenomeⅢ.Chloroplast DNA Evolution

Introduction

Materials and Methods

Plant Material

Purification of Chloroplasts

Preparation of Chloroplast DNA

Restriction Enzyme Analysis of Chloroplast DNA

Results and Discussion

Conclusions

Acknowledgement

References

Nicotiana Chloroplast Genome V.Construction，Mapping and Expression of Clone Library of N.otophora Chloroplast DNA

Introduction

Materials and Methods

Results

Cloning and Construction of N.otophora Chloroplast Genome Library

Mapping of Smal and BamHI Restriction Sites Map of N.otophora Chloroplast DNA

Localization of the Genes for Ribosomal RNA and the LS of RuBPCase

Expression of Nicotiana Chloroplast Genes in vivo

Discussion

Acknowledgements

References

Nicotiana chloroplast genome 7.Expression in E.coli and B.subtilis of tobacco and Chlamydomonas chloroplast DNA sequences coding for the large subunit of RuBP carboxylase

References

Light-Induced Transformation of Amyloplasts into Chloroplasts in Potato Tubers

Materials and Methods

Results

Discussion

Acknowledgments

LITERATURE CITED

Phytochrome Control of Levels of mRNA Complementary to Plastid and Nuclear Genes of Maize

Materials and Methods

Growth of Plants

Light Treatment

Extraction of RNA

Isolation of Chloroplast and Plasmid DNA

Radioactive Labeling of DNA

Dot Blots

Northern Blots

Hybridization

Results

Increase in Maize Plastid Photogene RNAs under Continuous or Intermittent Illumination

Phytochrome Control of the Levels of Plastid and Nuclear Photogene RNAs

Dot Blots

Northern Blots

Discussion

Acknowledgmems

Literature cited

The Organization of the Maize Plastid Chromosome：Properties and Expression of Its Genes

The Organization of the Plastid Chromosome

Features of Plastid Genes

Molecular Developmental Biology of Plastids

In Vitro Transcription of Chloroplast Genes

COMMENTS

Acknowledgments

REFERENCES

Effects of Light，Oxygen，and Substrates on Steady-State Levels of mRNA Coding for Ribulose-1，5-Bisphosphate Carboxylase and Light-Harvesting and Reaction Center Polypeptides in Rhodopseudomonas sphaeroides

Materials and Methods

Bacterial strains，media，and growth conditions

RuBPCase，protein，and bacteriochlorophyll assay

Extraction of RNA

Isolation of chromosomal DNA

Dot blots and Northern and Southern hybridizations

Chemicals

Results

Light-，O2-，and substrate-regulated levels of mRNA coding for RuBPCase，LH-1，RC-L，and RC-M

Dot hybridization

Northern hybridization

Southern hybridization

Discussion

Acknowledgments

References

Origin of the mRNA Stoichiometry of the puf Operon in Rhodobacter sphaeroides

Experimental Procedures

Bacterial Strains and Cell Growth

Extraction of RNA，Dot Blots，and Northern Hybridization

S-1 Nuclease Protection Analysis

Primer Extension Assay

Materials

Results

Effects of Light and Oxygen on the Levels of mRNAs Specifying LH-Ⅰ，RC-L，and RC-M

Differential Decay of the Large and Small puf Operon Transcripts

Determination of the 5'-Ends of the 2.6-and 0.5-kb puf Operon Transcripts by S-1 and Primer Extension Analysis

Differential Hybridization of the puf Operon Transcripts with Specific Deoxyoligonucleotide Probes

Discussion

References

Regulation of Expression of Genes for Light-Harvesting Antenna Proteins LH-I and LH-Ⅱ；Reaction Center Polypeptides RC-L，RC-M，and RC-H；and Enzymes of Bacteriochlorophyll and Carotenoid Biosynthesis in Rhodobacter Capsulatusby Light and Oxygen

Materials and Methods

Bacterial Strains，Media，and Growth Conditions

Preparation of Plasmid，M13 Phage，and Oligonucleotide Probes

RNA Isolation and Blot Hybridization

Results

Genes for LH-I and LH-Ⅱ

Genes for RC L，M，and H Polypeptides

Genes for Biosynthesis of Bchl and Crt

Discussion

References

Oxygen-Regulated mRNAs for Light-Harvesting and Reaction Center Complexes and for Bacteriochlorophyll and Carotenoid Biosynthesis in Rhodobacter capsulatus during the Shift from Anaerobic to Aerobic Growth

Material and Methods

Bacterial Strains and Culture Conditions

Pigment Extraction and Chromatophore Preparation

Extraction and Thin-Layer Chromatography of Carotenoids

Plasmid，M13 Phage，and Synthetic Oligonucleotide Probes

RNA Extraction，Dot Blots，and Northern Hybridization

Chemicals

Results

Absorption Spectra of Pigments and Chromatophores under Different Oxygen Concentrations

Decay of rRNA and mRNAs for LH-I and LH-Ⅱ Polypeptides and the Effects of Oxygen

Decay of mRNAs for RC-L，RC-M，and RC-H and the Effect of Oxygen

Decay of mRNAs for Bacteriochlorophyll and Carotenoid Biosynthesis and Oxygen Regulation

Discussion

Acknowledgments

References

Oxygen and Light Regulation of Expression of Genes for Light Harvesting（LH-Ⅰ，LH-Ⅱ），Reaction Center（RC-L，RC-M，RC-H），Pigment Biosynthesis and a Transcriptional Role in the Protective.Function of Carotenoids in Rhodobacter Capsulatus

Materials and Methods

Results and Discussion

References

Transcription of Oxygen-Regulated Photosynthetic Genes Requires DNA Gyrase in Rhodobacter Capsulatus（Supercoiling/DNA Conformation/Oxygen Regulation）

Materials and Methods

Bacteria and Anaerobic and Aerobic Growth Conditions

Assay of Pigments and Chromatophores

RNA Isolation and Blot Hybridization

Results

Differential Inhibition by Novobiocin and Coumermycin of the mRNAs for LH and RC Subunits，Bchl and Crt Biosyn-thetic Enzymes，Ribulose-Bisphosphate Carboxylase（RuP2-Case；EC 4.1.1.39），and Cytochromes

Effects of Novobiocin and Coumermycin on the Biosynthesis of Bchl and LH-I and LHII Complexes

Discussion

References

第五篇　植物转基因和生物防治（1987—1988）

Separation of Protein Crystals from Spores of Bacillus thuringiensis by Ludox Gradient Centrifugation

References

第六篇　医药生物工程药物制造和分子诊断（1989—2006）

目录

目 录

目　录