Restriction Enzyme Analysis of Chloroplast DNA

ct-DNA was digested with EcoRI，HindI，BamHI，SmaI，or Xhol as directed by the supplier，New England Biolabs.The restriction fragments were separated by electrophoresis in a 0.8%or 1.5%agarose slab gel according to the procedure or Helling et al.（1974）.Ethidium bromide-stained bands were visualized with a model C-62 Chromatovue transilluminator（Ultra-violet Product.Inc.）.The EcoRI and HindⅢ single or double digest fragments of lambda phage DNA were used as molecular weight markers.(https://www.daowen.com)