Restriction Enzyme Analysis of Chloroplast DNA

Chloroplast DNA was digested with EcoRI，BamHI，HindⅢ，XhoI，PstI，KpnI，SmaI，or SalI as directed by the supplier，New England Biolabs.The restriction fragments were seperated by electrophoresis in a 0.8%or 1.5%agarose slab gel according to the procedure of Helling et al.（1974）.The fragments produced by PstI，KpnI，SmaI，and SalI were analyzed in 0.8%agarose slab gels.Ethidium bromide-stained bands were visualized with a Model C-62 Chromatovue transilluminator（Ultra-Violet Product，Inc.）.The EcoRI and HindⅢ single or double digest fragments of Lambda phage DNA were used as molecular weight markers.(https://www.daowen.com)