Nicotiana Chloroplast Genome V.Construction，Mappin...
Summary
A total of 27 out of 28 BamHI restriction fragments of N.otophora ct-DNA have been stably cloned into plasmid pBR322.Each cloned fragment was identified by its electrophoretic mobility.By using this cloned library and a second restriction enzyme，SmaI，a physical map of N.otophora ct-DNA，was also constructed.The genes for rRNA and the LS of RuBPCase have been localized in the clone and marked on the map.No unusual structural feature of Nicotiana ct-DNA was detected in this species.Attempts to perform the in vivo expression of the cloned fragments employing the plasmid-directed protein synthesis system in maxicells met with some success.It is clearly demonstrated that certain cloned ct-DNA fragments could direct the synthesis of chloroplast polypeptides in E.coli.(https://www.daowen.com)
