Light-Induced Transformation of Amyloplasts into C...
Abstract
The transformation of amyloplast into chloroplasts in potato（Solanum tuberosum L.）tuber tissue can be induced by light.Excised potato tuber discs illuminated with white light of 3，000 lux began to synthesize chlorophyll after a lag period of 1 day，and continued to synthesize chlorophyll for 3 weeks.In this paper we present evidence，based on ultracentrifugal sedimentation and immunoprecipitation，that the light mediated synthesis of Ribulose-l，5-bisphosphate carboxylase began 1 day after illumination with white light.
When illuminated the chloroplasts isolated from light-grown potato tuber tissue incorporated［35S］methionine into polypeptides，one of which has been identified as the large subunit of Ribulose-1，5-bisphosphate carboxylase.These chloroplasts are functional as determined by O2 evolution in the Hill reaction.
Received for publication November 9，1983 and in revised form January 19，1984(https://www.daowen.com)
Yu S.Zhu，Denise L.Merkle-Lehman，Shain D.Kung，Department of Biological Sciences，University of Maryland Baltimore County，Catonsville，Maryland 21228
此文发表于1984年“Genet Plant Physiol”75：142-145.
A great deal of data is now available on the biochemistry of the development of chloroplasts from etioplasts［6］.Much less is known about the development of chloroplasts from amyloplasts，a starch storing organelle，which exists in storage tissue，roots，and some callus.It is well known that potatoes turn green on exposure to light for several days.Not only does the study of the organelle transformation and light-regulated gene expression stimulate theoretical interest，but the greening of potato also has some practical importance，since the greening of the potato is accompanied by the formation of the poisonous alkaloid，solanine［5］.The morphological and ultrastructural changes in the development of chloroplasts from amyloplasts were described in several electron microscopic studies［3，11］.In this communication we present some biochemical evidence to show the function of chloroplasts from potato tuber based on the biosynthesis of Chl，RuBPCase［2］，and Hill activity.
