Northern Blots

Total leaf RNA was glyoxylated in 1 mol/L glyoxal，10 mmol/L Na-phosphate（pH 6.8）at 50℃for 1 h and separated electrophoretically on 1.2%agarose gel using TBE（89 mmol/L Tris，89 mmol/L boric acid，2.5 mmol/L EDTA，pH 8.3）as running buffer［9］.The denatured RNA in 2XSSC was transferred to Gene Screen by blotting.These‘Northem’blots were dried and baked in the same manner as the Gene Screen dot blots.(https://www.daowen.com)