Materials and Methods
Bacterial strains,media and growth conditions R.capsulatus RifR strain SB1003 was used throughout this study.Mutants KZR9A12(crtA-),KZR9F4(crtI-),UAR87(RC-H-)and SB1007(bchC-)were used where indicated.Light intensities are denoted as follows:high light(HL),low light(LL),dark(D);O2 conditions are denoted:high O2(O2),low O2(LO2).Cell culture conditions are previously described[12].
Absorption spectroscopy of pigments.See[13].
Preparation of plasmid,M13 phage and oligonucleotide probes.See[12].(https://www.daowen.com)
RNA isolation,dot blots and Northern hybridization.See[12].