References

References

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［2］Bartholemew，J.W.1980.Stains for microorganisms in smears，p.375-440.In G.Clark（ed.），Staining procedures.The Williams & Wilkins Co.，Baltimore.

［3］Cooksey，K.E.1971.The protein crystal toxin of Bacillus thuringiensis：biochemistry and mode of action，p.247-274.In H.D.Burges and N.W.Hussey（ed.），Biological control of insects and mites.Academic Press，Inc.（London），Ltd.，London.

［4］Fast，P.G.1972.The δ-endotoxin of Bacillus thuringiensis.Ⅲ.A rapid method for separating parasporal bodies from spores.J.Invertebr.Pathol 20：139-140.

［5］Goodman，N.S.，R.J.Gottfried，and M.H.Rogoff.1967.Biphasic system for separation of spores and crystals of Bacillus thuringiensis.J.Bacteriol.94：485.

［6］Haider，M.Z.，E.S.Ward，and D.J.Ellar.1987.Cloning and heterologous expression of an insecticidal-endotoxin gene from Bacillus thuringiensis var.aizawai IC1 toxic to both lepidoptera and diptera.Gene 52：285-290.

［7］Herrnstadt，C.，G.G.Soares，E.R.Wikox，and D.L.Edwards.1986.A new strain of Bacillus thuringiensis with activity against coleopteran insects.Bio/Technology 4：305-308.(https://www.daowen.com)

［8］Mahillion，J.，and J.Delcour.1984.A convenient procedure for the preparation of highly purified parasporal crystals of Bacillus thuringiensis.J.Microbiol.Methods 3：69-75.

［9］Nickerson，K.W.1980.Structure and function of Bacillus thuringiensis protein crystal.Biotechnol.Bioeng.22：1305-1333.

［10］Schmitt，J.M.，and R.G.Herrmann.1980.Fractionation of cell organelles in silica sol gradients.Methods Cell Biol.15：179-209.

［11］Sharpe，E.S.，K.W.Nickerson，L.A.Bulla，Jr.，and J.N.Aronson.1975.Separation of spores and parasporal crystals of Bacillus thuringiensis in gradients of certain X-ray contrasting agents.Appl.Microbiol.30：1052-1053.

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[1]Yu Sheng Zhu，Allan Brookes，Ken Carlson，Philip Filner，Sungene Technologies Corporation，San Jose，California 95131-1818