A Simplified Method for Quantitation of Human Immu...
Summary
Human immunodeficiency virus type 1（HIV1）RNA was quantitated in the plasma of HIV1-seropositive patients using a simplified guanidinum-based extraction technique，reverse transcriptase（RT）and the polymerase chain reaction（PCR）.Plasma samples were obtained from 15 HIV1-seronegative individuals and 38 HIVl-seropositive patients.Following the extraction of RNA from plasma using“RNAzol”，HIV1 RNA was reversetranscribed using random hexamers，amplified by PCR and then detected by solution oligonucleotide hybridization，of the 15 HIV1-seronegative individuals，14 were negative for HIV1 RNA by RT-PCR.One high-risk patient who was HTLV-1-seropositive but HIVlseronegative was found to be positive for HIV1 RNA by RT-PCR.All 38 HIV1-seropositive patients were positive for HIV1 RNA by this technique.The HIV1 RNA levels in plasma varied from 800 to 500，000 copies/ml.patients with advanced clinical disease tended to have HIV1 RNA levels above 25，000 copies/ml.In patients studied serially，an increase in plasma HIV1 RAN correlated with a progressive decline in CD4+T cells and a deteriorating clinical course.The simplified quantitative RT-PCR assay for HIV1 RNA provides a useful tool for the evaluation and management of HIV disease.
Key-words：AIDS，ARC，HIV，RNA，RT，PCR；Quantitation，Clinical correlates，CD4 counts，AZT.
R.B.Stricker（1，2，3）（*），Y.S.Zhu（3）and B.F.Elswood（3）（1）HemaCare Corporation，San Francisco，CA，（2）Department of Medicine，California Pacific Medical Center，and（3）North American Biomedical Technologies Corporation，San Francisco，CA（USA）(https://www.daowen.com)
此文发表于1995年“Res.Virol”146：151-158.
（*）Address all correspondence to：Raphael B.stricker，HemaCare Corporation，450 sutter street，suite 1504，San Francisco，CA 94108，USA.
ARC=AlDS-related complex；AZT=azidothymidine（zidovudine）；ddC=dideoxycytidine；ddI=dideoxyinosine；DEPC=diethylpyrocarbonate；DNCB=dinitrochlorobenzene；LTR=long terminal repeat；PCR=polymerase chain reaction；RT=reverse transcriptase；Taq=Thermus aquaticus.
