孙晓玲（中国农业科学院茶叶研究所）
1.主要研究方向及内容
从事昆虫与茶树互作关系的化学与分子机制、利用化学生态学原理治理茶树害虫及抗性品种选育等方面的研究工作，重点揭示虫害诱导茶树防御反应以及害虫应对茶树防御反应的化学与分子机制。目前的研究方向主要有：茶树诱导防御反应的化学与分子机制及其利用；植食性昆虫适应茶树防御反应的化学与分子机制；茶树种质资源的抗性鉴定及抗性品种选育和创制。
2.代表性成果
茶树与灰茶尺蠖互作关系的化学与分子机制
儿茶素是茶叶中的关键风味物质和功能成分。对茶树儿茶素类化合物的研究多集中于生物合成、对非生物胁迫的响应，以及利用低温、遮阴等非生物胁迫方法调控茶树中儿茶素类化合物的含量从而改善茶叶品质等方面。目前，儿茶素已被证明是黑杨体内重要的诱导抗菌活性成分。然而，儿茶素是否是植物体内的诱导抗虫物质至今尚未见报道。因此，研究人员以此为切入点系统地研究了灰茶尺蠖幼虫取食为害对后续幼虫生长发育、儿茶素各组分含量和多种植物激素含量的影响；在明确差异儿茶素组分可显著降低灰茶尺蠖幼虫生长发育速率的基础上，深入探究了重要信号途径在其中的调控作用。研究结果表明，灰茶尺蠖幼虫取食通过诱导茶树儿茶素、表儿茶素和表没食子儿茶素、没食子酸酯含量的显著积累，最终产生直接防御反应；这一过程受茉莉酸、乙烯和植物生长素信号途径的共同调控（图12）。该项研究不仅建立了模拟灰茶尺蠖幼虫取食的标准化方法，而且极大地推动了对茶树与灰茶尺蠖互作关系的理解。
图12 灰茶尺蠖取食（灰色）和模拟取食（彩色）对植物激素含量的影响
3.代表性论文
Jing TT,Qian XN,Du WK,Gao T,Li DF,Guo DY,He F,Yu GM,Li SP,Schwab W,Wan XC,Sun XL,Song CK.2021.Herbivore-induced volatiles influence moth preference by increasing the β-Ocimene emission of neighbouring tea plants.Plant Cell Environ,44:3 667-3 680.
Ye M,Liu MM,Erb M,Glauser G,Zhang J,Li XW,Sun XL.2021.Indoleprimes defense signaling and increases herbivore resistance in tea plants.Plant Cell Environ,44(4):1 165-1 177.(https://www.daowen.com)
Zhang J,Sun XL.2020.Recent advances in polyphenol oxidase-mediated plant stress responses.Phytochemistry,181:112588.
Xu W,Dong YN,Yu YC,Xing YX,Li XW,Zhang X,Hou XJ,Sun XL.2020.Identification and evaluation of reliable reference genes for quantitativereal-time PCR analysis in tea plants under differential biotic stresses.SciRep-UK,10:2429.
Zhang J,Zhang X,Ye M,LiXW,Lin SB,Sun XL.2020.The jasmonic acid pathway positively regulates the polyphenol oxidase-based defense against tea geometrid caterpillars in the tea plant (Camellia sinensis).J Chem Ecol,4:308-316.
Lin SB,Dong YN,Li XW,Xing YX,Liu MM,Sun XL.2020.JA-Ile-Macrolactone 5b induces tea plant (Camellia sinensis) resistance to both herbivore Ectropis obliqua and pathogen Colletotrichum camelliae.Int J Mol Sci,21:1828.
Zhang X,Ran W,Liu FJ,Li XW,Hao WJ,Sun XL.2020.Cloning,expression and enzymatic characterization of a cystatin gene involved in herbivore defense in tea plant (Camellia sinensis).Chemoecology,1-12.https://doi.org/10.1007/s00049-020-00312-6 (IF=1.725).
Lin SB,Wang WW,Meng JP,Li XW,Wu J,Sun XL.2019.A transition metal-free approach to a regioselective total synthesis of the natural product derivative 6-methylellipticine,a potent anticancer agent.Tetrahedron Lett,https://doi.org/10.1016/j.tetlet.2019.151309 (IF=2.415).
Chen SL,Lu XT,Ge LG,Sun XL,Xin ZJ.2019.Wound- and pathogen- activated de novo JA synthesis using different ACX isozymes in tea plant (Camellia sinensis).J Plant Physiol,243:153047.https://doi.org/10.1016/ j.jplph.2019.153047 (IF=3.549).
Xin ZJ,Ge LG,Chen SL,Sun XL.2019.Enhanced transcriptome responses in herbivore-infested tea plants by the green leaf volatile (Z)-3-hexenol.J Plant Res,132(2):285-293 (IF=2.629).
Xin ZJ,ChenSL,Ge LF,Li XW,Sun XL.2019.The involvement of a herbivore-induced acyl-CoA oxidase gene,CsACX1,in the synthesis of jasmonic acid and its expression in flower opening in tea plant (Camellia sinensis).Plant Physiol Biochem,135:132-140 (IF=4.270).
