闫凤鸣（河南农业大学）
1.主要研究方向及内容
主要研究刺吸式昆虫与植物相互关系、病毒-介体-植物互作关系的化学生态学，以及棉铃虫和烟青虫的信息素生物合成、味觉感受和神经生物学。
2.代表性成果
病毒改变介体烟粉虱嗅觉敏感性的分子机制
2022年12月8日，团队在国际学术期刊Pest Management Science在线发表了题为A plant virus enhances odorant-binding protein 5 （OBP5）in the vector whitefly for more actively olfactory orientation to the host plant的研究论文。该研究以重要入侵害虫烟粉虱（Bemisia tabaci）及其半持久特异传播的瓜类褪绿黄化病毒（cucurbit chlorotic yellows virus，CCYV）为研究对象，揭示了CCYV对介体昆虫烟粉虱嗅觉系统的调控机制。携带植物病毒的介体昆虫的感觉和行为均有很大的不同，植物病毒可以通过直接（修饰介体昆虫的感觉和行为）和间接（改变植物外观、次生物质、营养、防御等）的方式改变介体的适合度和对寄主植物的适应性。该研究通过行为选择实验发现，感染CCYV的烟粉虱对寄主植物黄瓜具有更高的嗅觉灵敏性，具体表现为对黄瓜植株的选择比例增加以及反应时间的减少（图17）。利用转录组学技术筛选烟粉虱嗅觉相关基因的变化，结果表明，感染CCYV后，烟粉虱气味结合蛋白基因OBP5表达量显著提高。荧光定量qRT-PCR结果与其基本一致。说明CCYV的侵染对烟粉虱的嗅觉系统产生了影响。随后通过RNAi的方法对OBP5基因进行干扰实验，结果表明，干扰OBP5基因表达降低了烟粉虱对黄瓜植株的嗅觉灵敏性。为了进一步验证OBP5基因在烟粉虱寄主选择过程中的作用，通过GC-MS分析鉴定了黄瓜植株的挥发物成分，并通过Y形管实验进行烟粉虱的嗅觉行为反应测定。结果表明α-蒎烯、苯甲醛、柠檬烯以及β-罗勒烯对烟粉虱均有明显的吸引作用，感染CCYV的烟粉虱对其选择性出现不同程度的增强，相反，干扰OBP5基因表达会降低烟粉虱对不同化合物的选择反应。
图17 CCYV改变了介体烟粉虱的寄主定位行为
3.代表性论文(https://www.daowen.com)
He HF,Li JJ,Zhang ZL,Yan MH,Zhang BB,Zhu CQ,Yan WL,Shi BZ,Wang YX,Zhao CC,Yan FM.A plant virus enhances odorant-binding protein 5 (OBP5) in the vector whitefly for more actively olfactory orientation to the host plant.Pest Management Science,2022.https://doi.org/10.1002/ps.7313.
He HF,Zhao CC,Zhu CQ,Yan WL,Yan MH,Zhang ZL,Liu JL,Shi BZ,Bai RE,Li JJ,Yan FM.Discovery of novel whitefly vector proteins that interact with a virus capsid component mediating virion retention and transmission.International Journal of Biological Macromolecules,2022.https://doi.org/10.1016/j.ijbiomac.2022.11.229.
Yan MH,He HF,Zhang ZL,Zhang BB,Zhu CQ,Yan WL,Zhao CC,Li JJ,Yan FM.Molecular basis of mutual benefits between cucurbit chlorotic yellows virus (CCYV) transmission and imidacloprid resistance in Bemisia tabaci.Journal of Pest Science,2022.https://doi.org/10.1007/s10340-022-01553-w.
Zhang ZL,Zhang BB,He HF,Yan MH,Li JJ,Yan FM.Changes in visual and olfactory cues in virus-infected host plants alter the behavior of Bemisia tabaci.Frontiers in Ecology and Evolution,2022,10:766570.doi:10.3389/fevo.2022.766570.
Zhang ZL,He HF,Yan MH,Zhao CC,Lei CY,Li JJ,Yan FM.Widely targeted analysis of metabolomic changes of Cucumis sativus induced by Cucurbit chlorotic yellows virus.BMC Plant Biology,2022,22:158 https://doi.org/10.1186/s12870-022-03555-3.
He HF,Li JJ,Zhang ZL,Tang XF,Song DY,Yan FM.Impacts of Cucurbit chlorotic yellows virus (CCYV) on biological characteristics of its vector Bemisia tabaci (Hemiptera:Aleyrodidae) MED Species.Journal of Insect Science.2021.21(5):12; 1-7.https://doi.org/10.1093/jisesa/ieab084.
Lu SH,Li JJ,Bai RE,Yan FM.EPG-recorded feeding behaviors reveal adaptability and competitiveness in two species of Bemisia tabaci (Hemiptera:Aleyrodidae).Journal of Insect Behavior,2021,34(1):26-40.doi:10.1007/s10905-021-09765-1.
Lu SH,Chen MS,Li JJ,Shi Y,Gu QS,Yan FM.Changes in Bemisia tabaci feeding behaviors caused directly and indirectly by cucurbit chlorotic yellows virus.Virology Journal,2019,16:106.https://doi.org/10.1186/s12985-019-1215-8,ISSN:1743-422X.
