张真，孔祥波（中国林业科学研究院森林生态环境与保护研究所）
1.主要研究方向及内容
主要研究以松林、云杉林和杨树林为主的生态系统中害虫暴发机制和生态调控技术，重点研究对象包括松毛虫和小蠹虫等食叶害虫及蛀干害虫。以组学和化学生态学技术为研究手段，从害虫与寄主植物及环境关系入手，研究害虫发生及暴发的分子机制及化学生态学机制；同时针对我国重大生物灾害，研究精确、快速的自动化检测和监测技术，研发害虫可持续调控新技术；并结合人工智能、大数据等新技术及已有技术，形成害虫综合治理技术体系。主要研究内容包括：①昆虫分子生态及害虫分子调控技术开发：重点是松毛虫信息素感受及暴发的分子机制，同时研发对害虫的分子调控手段。②昆虫化学生态及信息化学物质应用技术：重点研究松毛虫和小蠹虫的种间化学生态学关系。开发重要的森林昆虫信息素，并研究其应用技术。③气候变化对森林害虫的影响及适应对策：重点研究森林生态系统及森林有害生物适应气候变化的机制和对策，探索气候变化背景下基于害虫为害机制的森林害虫的预测模型。
2.代表性成果
松林、云杉林和杨树林为主的生态系统中害虫暴发机制和生态调控技术
揭示了松毛虫暴发的化学和分子生态学机制；阐明嗅觉系统在昆虫信息素种间精确识别过程中的功能；从小蠹虫-伴生菌（或竞争者）-寄主之间的信息联系，揭示其暴发机制；利用RNAi或者基因编辑的方法开展森林昆虫重要基因功能研究，优化基因干扰效率，探索利用分子方法进行害虫种群控制；鉴定重要的舟蛾、尺蠖和小蠹虫的信息素，研究高效、高纯度的性信息素合成技术，开发相关信息素产品。研发林间监测和防治技术。
在“十三五”研究的基础上，将与相关公司合作研制的害虫自动远程监测仪与学科组所研制的信息素等技术结合，在全国范围内推广重要害虫的监测技术，提高森林害虫的监测准确性和科学性；针对以往的研究，形成以营造林措施为基础，及时准确监测为依据，以信息素等生物防治技术为手段的可持续生态调控技术，在有条件的典型地区进行推广应用。
图23 寄主-小蠹-天敌之间化学信号互作机制
3.代表性论文
Fang JX,Liu M,Zhang SF,Liu F,Zhang Z,Zhang QH,Kong XB.Chemical signal interactions of the bark beetle with fungal symbionts,and host/non-host trees.Journal of Experimental Botany,2020,71(19):6084-6091
Fang JX,Du HC,Shi X,Zhang SF,Liu F,Zhang Z,Zu PJ,Kong XB.Monoterpenoid signals and their transcriptional responses to feeding and juvenile hormoneregulation in bark beetle Ips hauseri.Journal of Experimental Biology,2021,224:jeb238030 (https://www.daowen.com)
Fang JX,Zhang SF,Liu F,Zhang Z,Cheng B,Zhang QH,Kong XB.Functional investigation of monoterpenes for improved understanding of the relationship between hosts and bark beetles.Journal of Applied Entomology,2021,145(4):303-331
Gan W,Kong XB,Fang JX,Shi X,Zhang SF,Li YX,Qu LJ,Liu F,Zhang Z,Zhang FB,Zhang XY.A pH-responsive fluorescent nanopesticide for selective delivery and visualization in pine wood nematode control.Chemical Engineering Journal,2023,463:142353
Liu F,Guo L,Zhang SF,Kong XB,Zhang Z.Synthesis and bioactivity of (13Z,15E)-octadecadienal:A sex pheromone component from Micromelalopha siversi Staudinger (Lepidoptera:Notodontidae).Pest Management Science,2021,77(1):264-272
Shen SF,Cao S,Zhang Z,Kong XB,Liu F,Wang GR,Zhang SF.Evolution of sex pheromone receptors in Dendrolimus punctatus Walker (Lepidoptera:Lasiocampidae) is divergent from other moth species.Insect Biochemistry and Molecular Biology,2020,122:103375
Shi X,Fang JX,Du HC,Zhang SF,Liu F,Zhang Z,Kong XB.Performance of two Ips bark beetles and their associated pathogenic fungi on hosts reflects a species-specific association in the beetle-fungus complex,Frontiers in Plant Science.2022,13(1029526)
Wang XJ,Liu F,Yun JP,Feng ZM,Jiang JS,Yang YN,Zhang PC.Iron-catalyzed synthesis of the hexahydrocyclopenta[c]furan core and concise total synthesis of polyfla vanostilbene B.Angewandte Chemie International Edition,2018,57(32):10127-10131
Wu NN,Zhang SF,Li XW,Cao YH,Liu XJ,Wang QH,Liu Q,Liu HH,Hu X,Zhou XG,James AA,Zhang Z,Huang YP,Zhan S.Fall webworm genomes yield insights into rapid adaptation of invasive species.Nature Ecology & Evolution,2019,3(1):105-115
Zhang X,Fan ZZ,Zhang R,Kong XB,Liu F,Fang JX,Zhang SF,Zhang Z.Bacteria-mediated RNAi for managing fall webworm,Hyphantria cunea :Screening target genes and analyzing lethal effect.Pest Management Science,2023,79:1566-1577
Zhang SF,Kong XB,Ze SZ,Wang HB,Lin AZ,Liu F,Zhang Z.Discrimination of cis-trans sex pheromone components in two sympatric Lepidopteran species.Insect Biochemistry and Molecular Biology,2016,73:47-54
Zhang SF,Shen SF,Peng J,Zhou X,Kong XB,Ren PP,Liu F,Han LL,Zhan S,Huang YP,Zhang AB,Zhang Z.Chromosome-level genome assembly of an important pine defoliator,Dendrolimus punctatus (Lepidoptera;Lasiocampidae).Molecular Ecology Resources,2020,20(4):1023-1037
