Isolation of Two Mutant Strains of Trichoderma Pse...
（1）Two mutants EA3-867 and N2-78 with high cellulase yields were obtained from the wild strains of Trichoderma pseudokoningii Rifai AS 3.3002 and Mo3 respectively by mutagenic treatments with various physical and chemical agents（high energy electrons，60Co，ultravioletray，N-methyl-N'-nitro-N-nitrosoguanid-ine，diethyl sulfate，etc.）.The cellulase activities of their koji，shake flask cultures，and enzyme preparations were distinctly higher than those of their parents.The mutant N2-78 reached quite high activity level when cultured in shake flasks on a simple medium containing milled straw，wheat bran，nutrient salts plus waste glucose molasses for 60 hours，and showed the highest cellulase saccharifying activities on CMC，filter paper and cotton，namely 255，8.2 and 13.4mg glucose/ml enzyme respectively，or 10.4 and 6 times more than those of its parent Mo3.Sueh cellulase activity levels are higher in comparation to the data reported in the literatures.
（2）The enzyme activities of cellulase preparations prepared from koji of EA3867 and N2-78 were compared with the Japanese commercial cellulase preparation“Onozuka R-10”.The cellulase activities of EA3-867 and N2-78 on CMC（saccharifying and liquefying activities），filter paper degradation，and filter paper and cotton saccharification were all higher than those of the“Onozuka R-10”，while pectinase activity was about the same.(https://www.daowen.com)
（3）Beside cellulases，the strains AS 3.3002，MO3，EA3-867 and N2-78 could also produce and secrete active pectinase.Hemicellulase，and some amylase and small amount of proteinase.In mutant strains not only the cellulase activities were enhanced，but pectinase was also inereased.
Cellulase Research Laboratory，Shanghai Institute of Plant Physiology，Academia Sinica and the Shanghai Distillery No.2
（4）As compared with the wild strains AS 3.3002 and Mo3，the mutants EA3-867 and N2-78 showed differences in the size，color and growth rate of the colonies，and in the production and morphology of conidia.They formed smaller colonies，grew more slowly，and produced less and longer conidia.But the lethal temperature for conidia of all the four strains was the same.
（5）The enzymatic characteristics of the mutants and wild strains were similar.The optimal pH and optimal temperature for the saccharification of CMC and of filter paper，and for filter paper degradation were 4.5 and 60℃，4.8 and 50℃，4.8 and 60℃respectively for all four strains.
（6）The method of mutation induction，assay and the levels of cellulase activities are discussed.
