Induction and Regulation of Cellulase Formation in...
A cellulase preparation of Trichoderma pseudokoningii Rafai N2-78，induced by sophorose，was fractionated by DEAE-Sephadex A-50 and Sephadex-100 column chromatography，and polyacrylamide gel electrophoresis.The components C1，Cx，andβglucosidase were obtained，and their molecular weights were estimated to be 67，000，62，000，and 42，000，respectively.The homogeneity of C1 component，which is a carbohydrate-protein complex and is rich in glycine，aspartic acid，threonine，serine，and glutamic acid，was verified by polyacrylamide gel and cellulose acetate film electrophoresis，immuno-electrophoresis and ultracentrifugal analysis.The C1 component showed a strong synergistic action with Cx andβ-glucosidase components in the degradation of cotton，avicel and Walthes cellulose.The hydrolysates of Walthes cellulose after incubation with C1 contained mainly cellobiose and a trace of glucose.The stability of these components to high temperature and different pH has also been studied.(https://www.daowen.com)
[1]Zhu Yu-sheng（Chu Yu-shung），Wu Yong-qiang，Chen Wei，Gao Jin-hua，Fei Jin-xin（Shanghai Institute of Plant Physiology'，Academia Sinica）
