Extraction of RNA

About 10 g of leaves were harvested from the seedlings under a dim green safe light and ground in a mortar with liquid N2.The frozen powder was suspended in 25 ml of hot lysis buffer（0.2 mol/L sodium borate，30 mmol/L EGTA，2%（w/v）sodium triisopropylnaphthalenesulfonate，pH 9.0）and was extracted with phenol/chloroform by the procedure previously described［9］.After repeated precipitation with alcohol and centrifugation，the RNA pellet was resuspended in 2 mol/L LiCl.The final pellet was dissolved in distilled H2O and analyzed spectrophotometrically and by agarose gel electrophoresis.(https://www.daowen.com)