Isolation of Chloroplast and Plasmid DNA
cpDNA was prepared as described previously[9].Plasmid DNAs were isolated and purified by cesium chloride centrifugation.The designations of the cloned plasmids used for probing transcripts of plastid and nuclear genes of maize are listed in Table 1 together with the sizes of the cloned inserts and the genes encoded.(https://www.daowen.com)