Dot blots and Northern and Southern hybridizations

Dot blots and Northern and Southern hybridizations

Dot blots and Northern and Southern hybridizations were performed as previously described［4］.The plasmids used as probes for hybridization are listed in Table 1.To quantitatively assess mRNA in either the dot blots or Northern hybridizations，the films were scanned with a GS300 transmittance reflectance scanning densitometer（Hoefer Scientific Instruments）linked to a recording integrator（Waters M 730 data module）.For quantitative comparisons，the dots on the filters were sometimes cut out and counted in 2，5-diphenyloxazole-1，4-bis-（5-phenyloxazolyl）benzene cocktail with a Beckman liquid scintillation counter.(https://www.daowen.com)