Chemicals

The DNA from plasmids pLI10，C-2-C，pUI135，and pUI128 were generous gifts of P.Hallenbeck and S.Kuhl，respectively.The DNA from plasmid pJW1 and cloned KpnI-PstI，AluI，Pvull-SalI，and XhoI-NruI fragments derived from pJWl were kindly supplied by P.Kiley.［y-32P］ATP was made by T.J.Donohue by the Johnson and Walseth technique［15］.RuBP was obtained from Sigma Chemical Co.，St.Louis，Mo.NaH14Co3（53 mCi/mmol）was obtained from New England Nuclear Corp.，Boston，Mass.Agarose，restriction enzymes，and a nick translation reagent kit were purchased from Bethesda Research Laboratories.Gene Screen（New England Nuclear）was used for all hybridizations.Proteinase K was purchased from Boehringer Mannheim Biochemicals，Indianapolis，Ind.RNase-free DNase I was obtained from Worthington Diagnostics，Freehold，N.J.Phenol was redistilled，and all other chemicals were reagent grade.(https://www.daowen.com)