Light-，O
2-，and substrate-regulated levels of mRNA coding for RuBPCase，LH-1，RC-L，and RC-M
Since RuBPCase levels and Bchl content are regulated by light，O2，and substrates，these parameters were varied to learn more about the nature of gene regulation in R.sphaeroides.The RNA isolated from cells grown under the different conditions was hybridized with 32P-labeled DNA probes for genes specifying RuBPCase，LH-1，RC-L，and RC-M，and semiquantitative measurements were made by dot hybridization.The Northern hybridization procedure affords additional information about the numbers and sizes as well as the amounts of the transcripts.
