RNA Isolation and Blot Hybridization

Total RNA was extracted as described［8］.After purification，the RNA was fractionated by electrophoresis and transferred to the mem-branes.Procedures for dot blots and RNA transfer hybrid-izations and the preparation of hybridization probes（plasmid DNA，M13 phage DNA，and synthetic oligonucleotides）for various photosynthetic genes have been described［9，10］.To quantify hybridization，either the autoradiograms were scanned with a GTS 300 transmittance-reflectance scanning densitometer（Hoefer，San Francisco）or the filters were assayed for radioactivity in 2，5-diphenyloxazole-1，4-bis（5-phenyloxazolyl）benzene cocktail with a Packard model 3385 liquid scintillation counter.(https://www.daowen.com)