1.2.5　滴加荧光二抗

goat anti-chicken IgG antibody coupled with Alexa Fluor 488（Invitrogen，Carlsbad，A11039，CA，1∶1000）和donkey anti-goat IgG antibody coupled with Alexa Fluor 594（Invitrogen，Carlsbad，A11058，CA，1∶1000），室温避光孵育60min；PBS冲洗，5min×3次。(https://www.daowen.com)