3.4 Identification and expression analysis of epig...
Epigenetic modifiers，of both histone methylation and acetylation，have played important roles in the regulation of secondary metabolism(Williams et al.，2008).9 HMTs，18 histone acetyltransferases(HATs)，and 13 HDACs were identified in each endophyte，across hy4 and Cg01.Among the HATs，12 GNATs(Gcn5-related Nacetyltransferases)were predicted in hy4 and Cg01.HDACs include conventional(Zndependent ClassⅠ，Ⅱ，andⅣprotein lysine deacetylases)and NAD+_dependent deacetylases.In both fungi，6 conventional and 7 NAD+_dependent HDACs were identified.(https://www.daowen.com)
The expression of HMT，HAT，and HDAC orthologues between hy4 and Cg01 was analyzed in each fungus.In HMT orthologous groups，only PpClr4-CgClr4 were expressed and showed a similar pattern both were downregulated after induction.For HATs，only PpSpt10-1-CgSpt10 were downregulated.In another HAT orthologous group PpSAS-2-CgSAS-2，CgSAS-2 was downregulated with q-value≤0.05，while PpSAS-2 was downregulated with p-value≤0.05.Among the HDAC DEGs，there was no HDAC orthologous group expressed with a similar pattern.In the groups of Pp-Clr3-CgClr3，PpSir2-7-CgSir 2-3，and PpSir 2-6-Cg Sir 2-6，the changes in HDAC expression in hy4 were opposite to those in Cg01.Because both deletion and overexpression of HDAC could result in SM accumulation with the same tendency(Studt et al.，2013)，the HDAC DEGs with opposite patterns in hy4 and Cg01 were listed as candidate genes(CgClr3，CgSir2-3，CgSir2-6).