《亚健康干预技术研究：园艺植物功能因子高值化利用》简介
《亚健康干预技术研究：园艺植物功能因子高值化利用》这本书是由.刘东波，熊兴耀编著创作的,《亚健康干预技术研究：园艺植物功能因子高值化利用》共有314章节
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Genomic characterization provides new insights int...
Xincong Kang1，2，3# Chichuan Liu4# Pengyuan Shen2，3 Liqin Hu2，3 Runmao Lin4Ji
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1 Introduction
(—)-Huperzine A(Hup A，［(5R，9R，11E)-5-amino-11-ethylidene-5，6，9，10-tetrahydro-7-m
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2.1 Fungal strains
The endophytic fungi P.polonicum hy4 and C.gloeosporioides Cg01 that produces Hu
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2.2 Extraction of genomic DNA and total RNA for se...
P.polonicum hy4 and C.gloeosporioides Cg01 were cultured in potato dextrose brot
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2.3 Sequencing and assembly
The genomes of P.polonicum hy4 and C.gloeosporioides Cg01 were sequenced with Il
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2.4 Gene annotation and phylogenetic analysis
To increase the accuracy of gene prediction，GeneMark-ES v4.32 and AUGUSTUS v2.7
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2.5 Differentially expressed gene analysis
Differential expression analysis of two groups(three biological replicates per g
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2.6 Gene-knockout and retro-complementation
The target genes were deleted by the split-marker homologous recombination knock
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2.7 High performance liquid chromatography(HPLC)an...
Strains were cultured as described above，and three replicates of culture media w
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3.1 Genome sequencing，assembly，and characterizatio...
The assembled genome of hy4 was 33.92Mb，and the total length of the eight larges
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3.2 RNA sequencing，identification and functional a...
About 4 Gb clean reads(removing sequencing adapters and low-quality bases)were g
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3.3 Identification and expression of the genes in ...
Because the genes LDC，CAO，PKS，BBE，P450 and 2OGD had been proposed to participate
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3.4 Identification and expression analysis of epig...
Epigenetic modifiers，of both histone methylation and acetylation，have played imp
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3.5 Functional analysis of candidate genes in HupA...
Gene deletion was conducted to analyze the association between the candidate gen
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SMRT sequencing revealed mitogenome characteristic...
Xincong Kang1，2，3# Liqin Hu2，3# Pengyuan Shen2，3 Rui Li4 Dongbo Liu1，2，3，5*
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1 Introduction
Ophiocordycepssinensis(syn.Cordycepssinensis)is an entomopathogenic fungus that
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2.1 Sample collection
Sample of the fungus O.sinensis(CCTCC AF 2017003)was from the fruiting body of t
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2.2 DNA extraction and genome sequencing
Total DNA was extracted from the stroma of O.sinensis by a modified CTAB method
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2.3 Assembly of the mitochondrial genome
Clean data were obtained by filtering out the sequencing adapters and low-qualit
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2.4 Mitochondrial genome annotation
Genes in O.sinensis mt genome were predicted by MFannot，RNAweasel(http：//megasun
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2.5 Analysis of repetitive sequences
Local BLASTn search of mtDNA against itself was performed using a cut-off e-valu