The genome of Ganoderma lucidum provides insights ...
Dongbo Liu1，2，* Jing Gong3，* Wenkui Dai4，* Xincong Kang1，2，* Zhuo Huang5 Hong-Mei Zhang3 Wei Liu3 Le Liu4 Junping Ma4 Zhilan Xia1，2 Yuxin Chen1，2 Yuewen Chen1，2 Depeng Wang6，7 Peixiang Ni4 An-Yuan Guo3，§ Xingyao Xiong1，8，§
1.Hunan Agricultural University，Changsha，410128，China；
2.State Key Laboratory of Sub-health Intervention Technology，Changsha，410128，China；
3.Hubei Bioinformatics& Molecular Imaging Key Laboratory，Department of Systems Biology，College of Life Science and Technology，Huazhong University of Science and Technology，Wuhan，430074，China；
4.Beijing Genome Institute(BGI-Shenzhen)，Shenzhen，518083，China；
5.Hunan Wanyuan Bio-tech Co.，Ltd.，Changsha，410128，China；(https://www.daowen.com)
6.Nextomics Biosciences Co.，Ltd.，Wuhan，430074，China；
7.School of Bioscience & Bioengineering，South China University of Technology，Guangzhou，510006，China；
8.Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province，Hunan Agricultural University，Changsha，410128，China)
Abstract：Ganoderma lucidum(Reishi or Ling Zhi)is the most famous traditional Chinese medicine and is widely used in the treatment of various human diseases in Asia countries.It is also a fungus with strong wood degradation ability with potential in bioenergy production.However，genes，pathways and mechanisms of these functions are still unknown.The genome of G.lucidum was sequenced and assembled into a 39.9Mb draft genome，which encoded 12080 protein-coding genes and～83% of them were similar with public sequences.After comprehensive annotation and comparison，genes in the biosynthesis of the main G.lucidum active ingredients，ganoderic acids(GAs)，were characterized.Among the GAs synthases，we identified a fusion gene，the N and C terminal of which are homologous to two different enzymes.Moreover，the fusion gene was only found in basidiomycetes.As a white rot fungus with wood degradation ability，abundant carbohydrate-active enzymes and ligninolytic enzymes were identified in the G.lucidum genome and were compared with other fungi.The genome sequence and well annotation of G.lucidum will provide new insights in function analyses including its medicinal mechanism.The characterization of genes in the triterpene biosynthesis and wood degradation will facilitate bioengineering research in the production of its active ingredients and bioenergy.
Keywords：Ganoderma lucidum，genome，triterpene synthesis，wood degradation