3.1 XKP decreasedβ-cell apoptotic rate under high glucose stress
Theβ-cell apoptotic rate in the high glucose concentration treatment(23.24%)was greater than that in the control(5.37%).Where XKP was added,the apoptotic rate was lower than the glucose-only treatment at 10.92%(Figure 1a,b).
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Figure 1
(a)Flow cytometry analysis of MIN-6 cells grown in different treatments of basic medium(control),basic medium with 33.3mmol/L glucose,basic medium with 33.3mmol/L glucose and 1μL/m L XKP mixture for 36h.(b)Statistical results of cell apoptosis rate.At least three replicates were performed.Error bars,s.d.;P<0.01 compared to control cells by one-way ANOVA.