2.6 Statistical analysis
The qRT-PCR data were analyzed by Livak 2—ΔΔCt method[39].Experimental result showed as mean±standard deviation(SD).Statistical differences between the groups were analyzed by using the one-way analysis of variance(ANOVA).A value of p<0.05 was considered significant and p<0.01 represented highly significant.(https://www.daowen.com)