参考文献
［1］Cepko C，Intrinsically different retinal progenitor cells produce specific types of progeny.Nat Rev Neurosci，2014，15（9）：615-627.
［2］Cepko C L，The determination of rod and cone photoreceptor fate.Annu Rev Vis Sci，2015，1：211-234.
［3］Eiraku M，Takata N，Ishibashi H，et al.Self-organizing optic-cup morphogenesis in threedimensional culture.Nature，2011，472（7341）：51-56.
［4］Lamba D A，Karl M O，Ware C B，et al.Efficient generation of retinal progenitor cells from human embryonic stem cells.Proc Natl Acad Sci U S A，2006，103（34）：12769-12774.
［5］Osakada F，Ikeda H，Mandai M，et al.Toward the generation of rod and cone photoreceptors from mouse，monkey and human embryonic stem cells.Nat Biotechnol，2008，26（2）：215-224.
［6］Meyer J S，Shearer R L，Capowski E E，et al.Modeling early retinal development with human embryonic and induced pluripotent stem cells.Proc Natl Acad Sci U S A，2009，106（39）：16698-16703.
［7］Phillips M J，Wallace K A，Dickerson S J，et al.Blood-derived human iPS cells generate optic vesicle-like structures with the capacity to form retinal laminae and develop synapses.Invest Ophthalmol Vis Sci，2012，53（4）：2007-2019.
［8］Zhong X，Gutierrez C，Xue T，et al.Generation of three-dimensional retinal tissue with functional photoreceptors from human iPSCs.Nat Commun，2014，5：4047.
［9］Cowan C S，Renner M，De Gennaro M，et al.Cell types of the human retina and its organoids at single-cell resolution.Cell，2020，182（6）：1623-1640.
［10］Onyak J R，Vergara M N，Renna J M.Retinal organoid light responsivity：current status and future opportunities.Transl Res，2022，250：98-111.
［11］Li L，Zhao H，Xie H，et al.Electrophysiological characterization of photoreceptor-like cells in human inducible pluripotent stem cell-derived retinal organoids during in vitro maturation.Stem Cells，2021，39（7）：959-974.
［12］Peng Y R，Shekhar K，Yan W，et al.Molecular classification and comparative taxonomics of foveal and peripheral cells in primate retina.Cell，2019，176（5）：1222-1237.
［13］Hu Y，Wang X，Hu B，et al.Dissecting the transcriptome landscape of the human fetal neural retina and retinal pigment epithelium by single-cell RNA-seq analysis.PLoS Biol，2019，17（7）：e3000365.
［14］Lu Y，Shiau F，Yi W，et al.Single-cell analysis of human retina identifies evolutionarily conserved and species-specific mechanisms controlling development.Dev Cell，2020.
［15］Welby E，Lakowski J，Di Foggia V，et al.Isolation and comparative transcriptome analysis of human fetal and iPSC-derived cone photoreceptor cells.Stem Cell Reports，2017，9（6）：1898-1915.
［16］Deng W L，Gao M L，Lei X L，et al.Gene correction reverses ciliopathy and photoreceptor loss in iPSC-derived retinal organoids from retinitis pigmentosa patients.Stem Cell Reports，2018，10（6）：2005.
［17］Buskin A，Zhu L，Chichagova V，et al.Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa.Nat Commun，2018，9（1）：4234.
［18］Tannenbaum S E，Turetsky T T，Singer O，et al.Derivation of xeno-free and GMP-grade human embryonic stem cells—platforms for future clinical applications.PLoS One，2012，7（6）：e35325.
［19］Lakowski J，Welby E，Budinger D，et al.Isolation of human photoreceptor precursors via a cell surface marker panel from stem cell-derived retinal organoids and fetal retinae.Stem Cells，2018，36（5）：709-722.
［20］Collin J，Zerti D，Queen R，et al.CRX expression in pluripotent stem cell-derived photoreceptors marks a transplantable subpopulation of early cones.Stem Cells，2019，37（5）：609-622.
［21］Zerti D，Hilgen G，Dorgau B，et al.Transplanted pluripotent stem cell-derived photoreceptor precursors elicit conventional and unusual light responses in mice with advanced retinal degeneration.Stem Cells，2021，39（7）：882-896.
［22］Chao J R，Lamba D A，Klesert T R，et al.Transplantation of human embryonic stem cell-derived retinal cells into the subretinal space of a non-human primate.Transl Vis Sci Technol，2017，6（3）：4.
［23］Mandai M，Fujii M，Hashiguchi T，et al.iPSC-derived retina transplants improve vision in rd1 end-stage retinal-degeneration mice.Stem Cell Reports，2017，8（1）：69-83.
［24］Iraha S，Tu H Y，Yamasaki S，et al.Establishment of immunodeficient retinal degeneration model mice and functional maturation of human Esc-derived retinal sheets after transplantation.Stem Cell Reports，2018，10（3）：1059-1074.
［25］Thomas B B，Lin B，Martinez-Camarillo J C，et al.Co-grafts of human embryonic stem cell derived retina organoids and retinal pigment epithelium for retinal reconstruction in immunodeficient retinal degenerate royal college of surgeons rats.Front Neurosci，2021，15：752958.
［26］Morrill S，He D Z Z.Apoptosis in inner ear sensory hair cells.J Otol，2017，12（4）：151-164.
［27］Roberts W M，Howard J，Hudspeth A J.Hair cells：transduction，tuning，and transmission in the inner ear.Annu Rev Cell Biol，1988，4：63-92.
［28］Schlosser G，Patthey C，Shimeld S M.The evolutionary history of vertebrate cranial placodes II.Evolution of ectodermal patterning.Dev Biol，2014，389（1）：98-119.
［29］Patthey C，Gunhaga L.Signaling pathways regulating ectodermal cell fate choices.Exp Cell Res，2014，321（1）：11-16.
［30］Trainor P A，Tam P P.Cranial paraxial mesoderm and neural crest cells of the mouse embryo：co-distribution in the craniofacial mesenchyme but distinct segregation in branchial arches.Development，1995，121（8）：2569-2582.
［31］Ding J，Tang Z，Chen J，et al.Induction of differentiation of human embryonic stem cells into functional hair-cell-like cells in the absence of stromal cells.Int J Biochem Cell Biol，2016，81（Pt A）：208-222.
［32］Chen F，Yang Y，Chen J，et al.Promotion of in vitro hair cell-like cell differentiation from human embryonic stem cells through the regulation of notch signaling.Metabolites，2021，11（12）.
［33］Koehler K R，Nie J，Longworth-Mills E，et al.Generation of inner ear organoids containing functional hair cells from human pluripotent stem cells.Nat Biotechnol，2017，35（6）：583-589.
［34］Menendez L，Trecek T，Gopalakrishnan S，et al.Generation of inner ear hair cells by direct lineage conversion of primary somatic cells.Elife，2020，9.
［35］Takahashi K，Yamanaka S.Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.Cell，2006，126（4）：663-676.
［36］Koehler K R，Mikosz A M，Molosh A I，et al.Generation of inner ear sensory epithelia from pluripotent stem cells in 3D culture.Nature，2013，500（7461）：217-221.
［37］Romano D R，Hashino E，Nelson R F.Deafness-in-a-dish：modeling hereditary deafness with inner ear organoids.Hum Genet，2022，141（3-4）：347-362.
［38］Nist-Lund C，Kim J，Koehler K R.Advancements in inner ear development，regeneration，and repair through otic organoids.Curr Opin Genet Dev，2022，76：101954.
［39］Malgrange B，Belachew S，Thiry M，et al.Proliferative generation of mammalian auditory hair cells in culture.Mechanisms of development，2002，112（1-2）：79-88.
［40］Oshima K，Grimm C M，Corrales C E，et al.Differential distribution of stem cells in the auditory and vestibular organs of the inner ear.Journal of the Association for Research in Otolaryngology，2007，8（1）：18-31.
［41］Li H，Liu H，Heller S.Pluripotent stem cells from the adult mouse inner ear.Nature medicine，2003，9（10）：1293-1299.
［42］Lang H，Xing Y，Brown L N，et al.Neural stem/progenitor cell properties of glial cells in the adult mouse auditory nerve.Scientific reports，2015，5（1）：1-21.
［43］Mc Lean W J，McLean D T，Eatock R A，et al.Distinct capacity for differentiation to inner ear cell types by progenitor cells of the cochlea and vestibular organs.Development，2016，143（23）：4381-4393.
［44］Chai R，Kuo B，Wang T，et al.Wnt signaling induces proliferation of sensory precursors in the postnatal mouse cochlea.Proceedings of the National Academy of Sciences，2012，109（21）：8167-8172.
［45］Sinkkonen S T，Chai R，Jan T A，et al.Intrinsic regenerative potential of murine cochlear supporting cells.Scientific reports，2011，1（1）：1-8.
［46］Barker N，Huch M，Kujala P，et al.Lgr5（+ve）stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro.Cell stem cell，2010，6（1）：25-36.
［47］Shi F，Hu L，Edge A S.Generation of hair cells in neonatal mice byβ-catenin overexpression in Lgr5-positive cochlear progenitors.Proceedings of the National Academy of Sciences，2013，110（34）：13851-13856.
［48］Chai R，Xia A，Wang T，et al.Dynamic expression of Lgr5，a Wnt target gene，in the developing and mature mouse cochlea.Journal of the Association for Research in Otolaryngology：JARO，2011，12（4）：455-469.
［49］Shi F，Kempfle J S，Edge A S B.Wnt-responsive Lgr5-expressing stem cells are hair cell progenitors in the cochlea.The Journal of Neuroscience：The Official Journal of The Society for Neuroscience，2012，32（28）：9639-9648.
［50］Cotanche D A.Regeneration of hair cell stereociliary bundles in the chick cochlea following severe acoustic trauma.Hearing Research，1987，30（2-3）：181-195.
［51］Cruz R M，Lambert P R，Rubel E W.Light microscopic evidence of hair cell regeneration after gentamicin toxicity in chick cochlea.Archives of Otolaryngology—head＆Neck Surgery，1987，113（10）：1058-1062.
［52］Bramhall N F，Shi F，Arnold K，et al.Lgr5-positive supporting cells generate new hair cells in the postnatal cochlea.Stem cell reports，2014，2（3）：311-322.
［53］Wang T，Chai R，Kim G S，et al.Lgr5+cells regenerate hair cells via proliferation and direct transdifferentiation in damaged neonatal mouse utricle.Nat Commun，2015，6：6613.
［54］Jan T A，Chai R，Sayyid Z N，et al.Tympanic border cells are Wnt-responsive and can act as progenitors for postnatal mouse cochlear cells.Development，2013，140（6）：1196-1206.
［55］Zhang S，Liu D，Dong Y，et al.Frizzled-9+supporting cells are progenitors for the generation of hair cells in the postnatal mouse cochlea.Frontiers in molecular neuroscience，2019，12：184.
［56］Zhang Y，Chen Y，Ni W，et al.Dynamic expression of Lgr6 in the developing and mature mouse cochlea.Frontiers in Cellular Neuroscience，2015，9：165.
［57］Zhang Y，Guo L，Lu X，et al.Characterization of Lgr6+cells as an enriched population of hair cell progenitors compared to Lgr5+cells for hair cell generation in the neonatal mouse cochlea.Frontiers in Molecular Neuroscience，2018，11：147.
［58］Lancaster M A，Knoblich J A.Organogenesis in a dish：modeling development and disease using organoid technologies.Science，2014，345（6194）：1247125.
［59］Kim W，Gwon Y，Park S，et al.Therapeutic strategies of three-dimensional stem cell spheroids and organoids for tissue repair and regeneration.Bioact Mater，2023，19：50-74.
［60］Hocevar S E，Liu L，Duncan R K.Matrigel is required for efficient differentiation of isolated，stem cell-derived otic vesicles into inner ear organoids.Stem Cell Res，2021，53：102295.
［61］Zhang Z，Gao S，Hu Y N，et al.Ti（3）C（2）T（x）MXene composite 3D hydrogel potentiates m TOR signaling to promote the generation of functional hair cells in cochlea organoids.Adv Sci（Weinh），2022，9（32）：e2203557.
［62］Gjorevski N，Lutolf M P.Synthesis and characterization of well-defined hydrogel matrices and their application to intestinal stem cell and organoid culture.Nat Protoc，2017，12（11）：2263-2274.
［63］Yue K，Trujillo-de Santiago G，Alvarez M M，et al.Synthesis，properties，and biomedical applications of gelatin methacryloyl（Gel MA）hydrogels.Biomaterials，2015，73：254-271.
［64］Suarez-Martinez E，Suazo-Sanchez I，Celis-Romero M，et al.3D and organoid culture in research：physiology，hereditary genetic diseases and cancer.Cell Biosci，2022，12（1）：39.
［65］Li H，Roblin G，Liu H，et al.Generation of hair cells by stepwise differentiation of embryonic stem cells.Proc Natl Acad Sci U S A，2003，100（23）：13495-13500.
［66］Koehler K R，Hashino E.3D mouse embryonic stem cell culture for generating inner ear organoids.Nat Protoc，2014，9（6）：1229-1244.(https://www.daowen.com)
［67］Yin X，Farin H F，van Es J H，et al.Niche-independent high-purity cultures of Lgr5+intestinal stem cells and their progeny.Nat Methods，2014，11（1）：106-112.
［68］Li H，Liu H，Heller S.Pluripotent stem cells from the adult mouse inner ear.Nat Med，2003，9（10）：1293-1299.
［69］Jeon S J，Fujioka M，Kim S C，et al.Notch signaling alters sensory or neuronal cell fate specification of inner ear stem cells.J Neurosci，2011，31（23）：8351-8358.
［70］Mc Lean W J，Yin X，Lu L，et al.Clonal expansion of Lgr5-positive cells from mammalian cochlea and high-purity generation of sensory hair cells.Cell Rep，2017，18（8）：1917-1929.
［71］Li X J，Doetzlhofer A.LIN28B/let-7 control the ability of neonatal murine auditory supporting cells to generate hair cells through m TOR signaling.Proc Natl Acad Sci U S A，2020，117（36）：22225-22236.
［72］Bok J，Zenczak C，Hwang C H，et al.Auditory ganglion source of Sonic hedgehog regulates timing of cell cycle exit and differentiation of mammalian cochlear hair cells.Proc Natl Acad Sci U S A，2013，110（34）：13869-13874.
［73］van der Valk W H，Steinhart M R，Zhang J，et al.Building inner ears：recent advances and future challenges for in vitro organoid systems.Cell Death Differ，2021，28（1）：24-34.
［74］Roccio M，Edge A S B.Inner ear organoids：new tools to understand neurosensory cell development，degeneration and regeneration.Development，2019，146（17）：dev177188.
［75］Zhang Y，Hao X L，Jia S F，et al.Efficient genetic engineering of murine cochlear organoids.J Tissue Eng Regen Med，2022，16（6）：530-537.
［76］Longworth-Mills E，Koehler K R，Hashino E.Generating inner ear organoids from mouse embryonic stem cells.Methods Mol Biol，2016，1341：391-406.
［77］Diensthuber M，Oshima K，Heller S.Stem/progenitor cells derived from the cochlear sensory epithelium give rise to spheres with distinct morphologies and features.J Assoc Res Otolaryngol，2009，10（2）：173-190.
［78］Jeong M，O'Reilly M，Kirkwood N K，et al.Generating inner ear organoids containing putative cochlear hair cells from human pluripotent stem cells.Cell Death Dis，2018，9（9）：922.
［79］DeJonge R E，Liu X P，Deig C R，et al.Modulation of Wnt signaling enhances inner ear organoid development in 3D culture.PLoS One，2016，11（9）：e0162508.
［80］Xu F，Yan W，Cheng Y.Pou4f3 gene mutation promotes autophagy and apoptosis of cochlear hair cells in cisplatin-induced deafness mice.Arch Biochem Biophys，2020，680：108224.
［81］Wallis D，Hamblen M，Zhou Y，et al.The zinc finger transcription factor Gfi1，implicated in lymphomagenesis，is required for inner ear hair cell differentiation and survival.Development，2003，130（1）：221-232.
［82］Matern M S，Milon B，Lipford E L，et al.GFI1 functions to repress neuronal gene expression in the developing inner ear hair cells.Development，2020，147（17）.
［83］Pan B，Géléoc G S，Asai Y，et al.TMC1 and TMC2 are components of the mechanotransduction channel in hair cells of the mammalian inner ear.Neuron，2013，79（3）：504-515.
［84］Fettiplace R.Is TMC1 the hair cell mechanotransducer channel？Biophys J，2016，111（1）：3-9.
［85］Ryu N，Sagong B，Park H J，et al.Screening of the SLC17A8 gene as a causative factor for autosomal dominant non-syndromic hearing loss in Koreans.BMC Med Genet，2016，17：6.
［86］Kubota M，Scheibinger M，Jan T A，et al.Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea.Cell Rep，2021，34（3）：108646.
［87］Zheng J，Shen W，He D Z，et al.Prestin is the motor protein of cochlear outer hair cells.Nature，2000，405（6783）：149-155.
［88］Butan C，Song Q，Bai J-P，et al.Single particle cryo-EM structure of the outer hair cell motor protein prestin.Nature Communications，2022，13（1）：290.
［89］Wu Z，Grillet N，Zhao B，et al.Mechanosensory hair cells express two molecularly distinct mechanotransduction channels.Nat Neurosci，2017，20（1）：24-33.
［90］Ratzan E M，Moon A M，Deans M R.Fgf8 genetic labeling reveals the early specification of vestibular hair cell type in mouse utricle.Development，2020，147（22）.
［91］Meyers J R，MacDonald R B，Duggan A，et al.Lighting up the senses：FM1-43 loading of sensory cells through nonselective ion channels.J Neurosci，2003，23（10）：4054-4065.
［92］Zhang J，Wang Q，Abdul-Aziz D，et al.ERBB2 signaling drives supporting cell proliferation in vitro and apparent supernumerary hair cell formation in vivo in the neonatal mouse cochlea.Eur J Neurosci，2018，48（10）：3299-3316.
［93］Lenz D R，Gunewardene N，Abdul-Aziz D E，et al.Applications of Lgr5-positive cochlear progenitors（LCPs）to the study of hair cell differentiation.Front Cell Dev Biol，2019，7：14.
［94］Liu Q，Zhang L，Zhu M S，et al.High-throughput screening on cochlear organoids identifies VEGFR-MEK-TGFB1 signaling promoting hair cell reprogramming.Stem Cell Reports，2021，16（9）：2257-2273.
［95］Mc Lean W J，Yin X，Lu L，et al.Clonal expansion of Lgr5-positive cells from mammalian cochlea and high-purity generation of sensory hair cells.Cell reports，2017，18（8）：1917-1929.
［96］Erni S T，Gill J C，Palaferri C，et al.Hair cell generation in cochlear culture models mediated by novel gamma-secretase inhibitors.Frontiers in Cell and Developmental Biology，2021，9.
［97］Tang P C，Alex A L，Nie J，et al.Defective Tmprss3-associated hair cell degeneration in inner ear organoids.Stem Cell Reports，2019，13（1）：147-162.
［98］Fukunaga I，Fujimoto A，Hatakeyama K，et al.In vitro models of GJB2-related hearing loss recapitulate Ca（2+）transients via a gap junction characteristic of developing cochlea.Stem Cell Reports，2016，7（6）：1023-1036.
［99］Chen J R，Tang Z H，Zheng J，et al.Effects of genetic correction on the differentiation of hair cell-like cells from iPSCs with MYO15A mutation.Cell Death Differ，2016，23（8）：1347-1357.
［100］Hosoya M，Fujioka M，Sone T，et al.Cochlear cell modeling using disease-specific iPSCs unveils a degenerative phenotype and suggests treatments for congenital progressive hearing loss.Cell Rep，2017，18（1）：68-81.
［101］Wang H，Luo Y，Li J，et al.Generation of a gene corrected human isogenic iPSC line（CPGHi001-A-1）from a hearing loss patient with the TMC1 p.M418K mutation using CRISPR/Cas9.Stem Cell Res，2022，60：102736.
［102］Lasorella A，Stegmuller J，Guardavaccaro D，et al.Degradation of Id2 by the anaphase-promoting complex couples cell cycle exit and axonal growth.Nature，2006，442（7101）：471-474.
［103］Izumikawa M，Minoda R，Kawamoto K，et al.Auditory hair cell replacement and hearing improvement by Atoh1 gene therapy in deaf mammals.Nat Med，2005，11（3）：271-276.
［104］Chen W，Jongkamonwiwat N，Abbas L，et al.Restoration of auditory evoked responses by human ES-cell-derived otic progenitors.Nature，2012，490（7419）：278-282.
［105］Ealy M，Ellwanger D C，Kosaric N，et al.Single-cell analysis delineates a trajectory toward the human early otic lineage.Proceedings of the National Academy of Sciences，2016，113（30）：8508-8513.
［106］Oshima K，Shin K，Diensthuber M，et al.Mechanosensitive hair cell-like cells from embryonic and induced pluripotent stem cells.Cell，2010，141（4）：704-716.
［107］Corrales C E，Pan L，Li H，et al.Engraftment and differentiation of embryonic stem cellderived neural progenitor cells in the cochlear nerve trunk：Growth of processes into the organ of corti.Journal of Neurobiology，2006，66（13）：1489-1500.
［108］Noda T，Meas S J，Nogami J，et al.Direct reprogramming of spiral ganglion non-neuronal cells into neurons：toward ameliorating sensorineural hearing loss by gene therapy.Frontiers in Cell and Developmental Biology，2018，6：16.
［109］Rivetti Di Val Cervo P，Romanov R A，Spigolon G，et al.Induction of functional dopamine neurons from human astrocytes in vitro and mouse astrocytes in a Parkinson's disease model.Nature biotechnology，2017，35（5）：444-452.
［110］Costa A，Sanchez-Guardado L，Juniat S，et al.Generation of sensory hair cells by genetic programming with a combination of transcription factors.Development，2015，142（11）：1948-1959.
［111］Eiraku M，Watanabe K，Matsuo-Takasaki M，et al.Self-organized formation of polarized cortical tissues from ESCs and its active manipulation by extrinsic signals.Cell stem cell，2008，3（5）：519-532.
［112］Koehler K R，Mikosz A M，Molosh A I，et al.Generation of inner ear sensory epithelia from pluripotent stem cells in 3D culture.Nature，2013，500（7461）：217-221.
［113］Litsiou A，Hanson S，Streit A.A balance of FGF，BMP and WNT signalling positions the future placode territory in the head.Development，2005，132（18）：4051-4062.
［114］Martin K，Groves A K.Competence of cranial ectoderm to respond to Fgf signaling suggests a two-step model of otic placode induction.Development，2006，133151：877-887.
［115］DeJonge R E，Liu X-P，Deig C R，et al.Modulation of Wnt signaling enhances inner ear organoid development in 3D culture.PloS one，2016，11（9）：e0162508.
［116］Liu X-P，Koehler K R，Mikosz A M，et al.Functional development of mechanosensitive hair cells in stem cell-derived organoids parallels native vestibular hair cells.Nature Communications，2016，7（1）：1-11.
［117］Koehler K R，Nie J，Longworth-Mills E，et al.Generation of inner ear organoids containing functional hair cells from human pluripotent stem cells.Nature biotechnology，2017，35（6）：583-589.
［118］Munnamalai V，Fekete D M.Building the human inner ear in an organoid.Nature Biotechnology，2017，35（6）：518-520.
［119］Locher H，Frijns J H，van Iperen L，et al.Neurosensory development and cell fate determination in the human cochlea.Neural Development，2013，8（1）：1-14.
［120］Roccio M，Perny M，Ealy M，et al.Molecular characterization and prospective isolation of human fetal cochlear hair cell progenitors.Nature Communications，2018，9（1）：1-14.
［121］Bok J，Dolson D K，Hill P，et al.Opposing gradients of Gli repressor and activators mediate Shh signaling along the dorsoventral axis of the inner ear.2007.
［122］Perny M，Ting C-C，Kleinlogel S，et al.，Generation of otic sensory neurons from mouse embryonic stem cells in 3D culture.Frontiers in Cellular Neuroscience，2017，11：409.
［123］Roccio M，Hahnewald S，Perny M，et al.Cell cycle reactivation of cochlear progenitor cells in neonatal FUCCI mice by a GSK3 small molecule inhibitor.Scientific Reports，2015，5（1）：1-11.
［124］Stockhausen M，Sjölund J，Manetopoulos C，et al.Effects of the histone deacetylase inhibitor valproic acid on Notch signalling in human neuroblastoma cells.British Journal of Cancer，2005，92（4）：751-759.
［125］Shi F，Kempfle J S，Edge A S.Wnt-responsive Lgr5-expressing stem cells are hair cell progenitors in the cochlea.Journal of Neuroscience，2012，32（28）：9639-9648.
［126］Rossi G，Manfrin A，Lutolf M P.Progress and potential in organoid research.Nat Rev Genet，2018，19（11）：671-687.
［127］Shirai H，Mandai M，Matsushita K，et al.Transplantation of human embryonic stem cellderived retinal tissue in two primate models of retinal degeneration.Proc Natl Acad Sci U S A，2016，113（1）：E81-90.
［128］Yui S，Nakamura T，Sato T，et al.Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5（+）stem cell.Nat Med，2012，18（4）：618-623.
［129］Fordham R P，Yui S，Hannan N R，et al.Transplantation of expanded fetal intestinal progenitors contributes to colon regeneration after injury.Cell Stem Cell，2013，13（6）：734-744.
［130］Assawachananont J，Mandai M，Okamoto S，et al.Transplantation of embryonic and induced pluripotent stem cell-derived 3D retinal sheets into retinal degenerative mice.Stem Cell Reports，2014，2（5）：662-674.
［131］Schwank G，Koo B K，Sasselli V，et al.Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients.Cell Stem Cell，2013，13（6）：653-658.
［132］Ishii M，Tateya T，Matsuda M，et al.Retrograde ERK activation waves drive base-to-apex multicellular flow in murine cochlear duct morphogenesis.Elife，2021，10.