HIV1 RNA Extraction

Extractions were performed on 100 μl aliquots of plasma.Total RNA was extracted from plasma using“RNAzol”（Biotecx，Houston，TX），an acidic guanidinium-thiocyanatebased reagent（Chomcynski and Sacchi，1987），according to the manufacturer's instructions，with the following modifications：2 μg of yeast tRNA was added to the plasma at the beginning of each extraction，and only one isopropanol precipitation of the total RNA was performed.Total RNA was dissolved and stored in DEPC-H2 O at-70℃.RNA samples stored in this manner have been shown to be stable for up to six months（Zhu et al.，1995）.(https://www.daowen.com)