2.1 Fungal strains
The endophytic fungi P.polonicum hy4 and C.gloeosporioides Cg01 that produces Hup A were isolated and screened from H.serrata,which collected from Nanping,Fujian,China.Two strains were purified by single-spore isolation and identified by ITS sequencing.The strains were cultured on Potato Dextrose Agar medium at 28℃and deposited at the China Center for Type Culture Collection(hy4:CCTCC No.M2010086,Cg01:CCTCC No.AF2018024;Wuhan,China).
Colony of P.polonicum hy4 on PDA was cyan,wrinkled,good sporulation,margin slightly irregular,reverse light brown(Figure 2).Colony of C.gloeosporioides Cg01 grown from single conidia on PDA about 8 cm diam after 5 d,aerial mycelium sparse,white,or grey-white,surface of colony with numerous acervuli,some with dark bases,with orange conidial ooze(Figure 2).(https://www.daowen.com)

Figure 2 Morphology of P.polonicum hy4(A)and C.gloeosporioides Cg01(B)on potato dextrose agar