2.2 Extraction of genomic DNA and total RNA for se...
P.polonicum hy4 and C.gloeosporioides Cg01 were cultured in potato dextrose broth(PDB)at 28℃for 3-5d.Mycelia were collected by filtering the liquid culture and used for genomic DNA extraction by a modified CTAB method as previously described(Kang，2011).
Total RNA was extracted from different samples of P.polonicum hy4 and C.gloeosporioides Cg01 using TRIzol(Takara，Dalian，China).Control group：either hy4 or Cg01 cultured in PDB enriched media(with added peptone 2g/L，yeast extract powder 2g/L，KH2 PO4 1.5g/L，MgSO4 0.5g/L，NaCl 0.3g/L in PDB，p H 6.5)for 12d at 28℃；induced group：either hy4 or Cg01 cultured in PDB enriched media for 5 d at 28℃，extracts of H.serrata were added，and subsequently cultured another 7d.P.polonicum hy4 only produced Hup A with the extract of H.serrata(535.54μg/L)，while C.gloeosporioides could produce Hup A in small amounts(21.04μg/L)alone and increased to 556.21μg/L with the extract of H.serrata.(https://www.daowen.com)
RNA purity and integrity were verified by electrophoresis on 1%agarose gel.RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system(Agilent Technologies，CA，USA).RNA quantity was measured using Qubit RNA Assay Kit in Qubit 2.0 Flurometer(Life Technologies，CA，USA).The RNA samples were sent to Novogene(Beijing，China)for RNA sequencing.