2.3　Predicted Gene models

By combining several different gene predictors(see methods)，we identified 12，080 protein-coding gene models，245 tRNA，1 rRNA and 15 snRNA with a total length of 17，343，729bp，accounting for 43.41% of the genome(Table 1).The gene density was 3.34 genes/10kb and the average gene size of protein coding genes was 1435bp，typically with small exons(average 230bp)and much smaller introns(average 100bp)，which were similar with other basidiomycetes［20］.There were average 6.25 exons in one gene.Notably，the G+C content in protein coding gene regions，which was 58.86%，was slightly higher than the whole genome(55.56%)and other basidiomycetes［20］.(https://www.daowen.com)

Among the 245 tRNA genes，10 tRNAs were pseudogenes and 141 tRNAs contained an intron.Forty six out of 61 possible anti-codon tRNA were found，corresponding to the codons of 20 amino acids.Except for several codons，the usage frequencies of most codons were proportional to the numbers of anti-codon proportion.For lacking of 18 anti-codons，we speculated anticodon repertoire in this genome was consistent with the normal wobble rules［22］which allows anticodon：codon pairings：I/ANN：NNU，NNC；GNN：NNU，NNC；UNN：NNA；CNN：NNG).