2.9 Function annotation of putative FOLymes.
To assess the degradation in the genomic level，the proteins of G.lucidum were aligned to proteins in the FOLy(Fungal Oxidative Lignin enzymes)database，which collected and classified enzymes involved in lignin catabolism.The FOLymes mainly comprise two families，lignin oxidases(LO families)and lignin-degrading auxiliary enzymes that generate H2 O2 for peroxidases(LDA families).G.lucidum contained a total of 37 members in FOLymes(23 genes in LO families and 14 genes in LDA families，Table 7)which was more than the brown-rot fungi F.pinicola，G.trabeum and the fungi without ligninolytic activity，such as Malassezia globosa，Pyrenophora tritici-repentis and Saccharomyces cerevisiae.In contrast，it had fewer FOLymes than the coprophilic fungus Coprinopsiscinerea(53 FOLymes)and the white-rot fungus Pleurotusostreatus(43 FOLymes).While G.lucidum had the largest number of lignin oxidases(LO families).The LO families can further divided into 3 subfamilies，which are laccases(LO1)，lignin peroxidases，manganese peroxidases，versatile peroxidases(LO2)and cellobiose dehydrogenases(CDHs；LO3).G.lucidum contained 15 laccase genes(LO1)，7 peroxidase(LO2)and 1 cellobiose dehydrogenase(LO3).For the seven peroxidases(LO2)genes in G.lucidum，two of them locate at scaffold 10 and 3 locate at scaffold 79 which maybe form a gene cluster.While LDA families have 7 sub families，G.lucidum contains 1 aryl-alcohol oxidase(LDA1)，10 copper radical oxidase(LDA3)，2 glucose oxidase(LDA6)and 1 benzoquinone reductase(LDA7).No genes were found in LDA2，LDA4 and LDA5 in G.lucidum as well as most of other fungi.The major fungi were rich in LO1，LDA1 and LDA3 except P.chrysosporium，which had no genes in LO1 but had 16 LO2 genes.
Table 7 The gene distribution of FOLymes in G.lucidum and other fungi(https://www.daowen.com)
The abbreviations of species were the same with Table 2.