Discussion

G.lucidum is one of the most famous traditional medicines in China and it is also an important fungus in cellulose and lignin degradation with potential ability in energy production.The genome sequencing and annotation of G.lucidum is crucial in its function and comparative genomics research.Here we selected the most commonly used G.lucidum in China and sequenced its genome sequence by Solexa technology.We assembled the sequences into 634 scaffolds in 39.9Mb sequences which represented about 94% of the whole genome and annotated 12，080 gene models at genome level.We noticed that JGI(DOE Joint Genome Institute)also have sequenced and annotated the genome of a North American isolate G.lucidum.The assembled scaffold size and predicted gene models of our sequenced genome were very similar with the JGI genome annotation，which indicated the quality of our sequence and annotation reliable.(https://www.daowen.com)

The G.lucidum genome is characteristics of relatively high GC content and less TEs compared with other sequenced fungi.In G.lucidum genome，we only identified 4.6%TE sequences of the genome，which was much less than other reported fungi，for example，Laccaria bicolor(21%)［20］and Tuber melanosporum(58%)［26］.To check whether the lower repeat percentage was caused by methodology or not，we have used our approaches to identify the repeat sequences in L.bicolor and found a similar percentage of repeat sequences reported［20］.This confirmed that the TEs in G.lucidum were really much less than other fungi.Although the repeat sequence percentage is different，the genome size，gene length，and gene annotation of G.lucidum are similar and comparable with other Basidiomycota fungi.