5.1　Strains and culture conditions

A fruiting body of G.lucidum was collected from oak at Hengshan，Hunan province，China，on May 21th，2001.It deposited at the edible fungi institute of Hunan agricultural university(Changsha，Hunan，China).(https://www.daowen.com)

Basidiospores from the fruiting body of G.lucidum were collected by hood.Single spores were separated by micro-manipulation of basidiospores and allowed to geminate on PDA(Potato Dextrose Agar)enrichment medium(20% potato，2% dextrose，0.2%yeast extract，0.2%peptone，0.3% monopotassium phosphate，0.15% magnesium sulfate and 2%agar)at 25℃，dark.Germination started 4～8 days after plating.After 3～5 days growth，clamp connection was observed by using optical microscopy.Forty-six single basidiospores with no clamp connection were isolated and transferred individually to fresh dishes，sealed and stored at 4℃after 10～15 days growth.For genomic DNA isolation，single basidiospore named P9 which grew well was cultured in PDA enrichment medium without agar at 25℃，dark，shaken at 120 r/min，for 8～10 days.