2.7　Isolation of peritoneal macrophages

Peritoneal macrophages were isolated as previously reported［18］.Briefly，peritoneal macrophages were collected from mice by peritoneal lavage with Dulbecco phosphatebuffered saline.Thioglycolate-elicited peritoneal cells were obtained from animals 3 days after intraperitoneal injection with 3mL thioglycolate broth(Sigma-Aldrich).Macrophages were further purified by adherence to culture dishes for 24h.(https://www.daowen.com)