2.1　Reagents and antibodies

GA I(D1924)was purchased from Shanghai Baoman Biological Technology Co.，Ltd(Shang Hai，China).Chloroquine(50-63-5)was from Sigma-Aldrich(St.Louis，MO).Caspase-3 activity assay kit(C1115)was from Beyotime Institute of Biotechnology Co.Ltd.(Beijing，China).Anti-LC3(light chain 3)polyclonal antibody(PM036)for immunofluorescence staining and(PM046)western blot analysis was obtained from Medical and Biological Laboratories(MBL；Japan).Anti-SQSTM1/p62 antibody(PM045)was from MBL.p70S6K(2708)and phospho-p70S6K(9206)were obtained from Cell Signalling Technology(Beverly，MA).Mammalian target of rapamycin(m TOR；2983)and phospho-mTOR(5536)were obtained from Cell Signalling Technology.Anti-GAPDH(glyceraldehyde 3-phosphate dehydrogenase)antibody(ZB002)was purchased from YTHX Biotechnology Co.，Ltd.(Beijing，China).Propidium iodide(PI)-annexin V/fluorescein isothiocyanate(FITC)for flow cytometry was purchased from Beyotime Institute of Biotechnology Co.Ltd.Goat anti-mouse IgG(1070-05)was from SouthernBiotech(Birmingham，UK).Goat anti-rabbit(4050-05)was purchased from SouthernBiotech.Alexa Fluor 488 Goat Anti-Rabbit(A11034)was from Invitrogen Life Technology(Shanghai，China).(https://www.daowen.com)