2.2　Cell culture

MIN-6 cells were cultured in Dulbecco's modified Eagle's medium(DMEM；8116008；Gibco Shanghai，China)supplemented with 10%fetal bovine serum(FBS；1507772；BI，Israel)and 1% 100×L-glutamine(TransGen；FG201-01，Beijing，China)in 37℃，5% CO2.(https://www.daowen.com)