Culture

The glycerol medium was used to produce mycelium in the induction study.［10］The medium consists of（%）：glycerol，0.6；（NH4）2SO4，0.14；KH2PO4，0.2；urea，0.03；MgSO4·7H2 O，0.03；CaCl2，0.03；peptone，0.1；micro elements（ppm）：FeSO4·7H2O，7；MnSO2·H2O，1.56；ZnCl2，1.67；CoCl2，2；pH=5.3.In an experiment designed to study cellulase induction，a variety of carbon sources were substituted for glycerol.1 ml of spore suspension（106）of Trichoderma was inoculated on 100 ml glycerol medium，and cultured in a reciprocating shaking bath（108 strokes per min）at 30℃for 48 h.Then，the mycelium was harvested on a filter paper by suction filtration and washed with 200 ml 0.01 M phosphate buffer（pH=5.0）.(https://www.daowen.com)