5.4　KEGG pathway analysis

To compare the pathway annotation of G.lucidum with other fungi，we also mapped genes in other fungal genomes to KEGG database.The Basidiomycota comprises three taxa(agaricomycotina，ustilaginomycotina and pucciniomycotina).The Ascomycota includes four taxa-eurotiomycetes，dothideomycetes，sordariomycetes and saccharomycotina.For comparative genome analysis，we selected 12 basidiomycetes，5 ascomycota and downloaded their genomic gene models.Then，we compared the number of genes in each KEGG terms among all these genomes.Terms in KEGGare divided into four layers.The first layer consists of seven sections，including“Metabolism”，“Genetic information processing”，“Environmental information processing”and so on.Each section is further divided into several small entries，which are the second layers.The third layer is the specific pathway map and the fourth layer includes specific genes in each pathway.We compared the gene distribution of these species in the second layer and investigated the sub-terms(e.g.KO in KEGG pathway)if the term had significant different gene numbers in the second layer.(https://www.daowen.com)

Because many P450 genes were involved in KEGG pathway，we compared P450 genes at the genomic level.All genome sequences were aligned to fungi P450 sequences in Cytochrome P450 database(cut-off e-value：1e-10 and identity＞30%).For glutathione S-transferase(GST)gene，we mapped all genomic genes to GSTs genes described in literature［24］，which classified fungal GSTs into different sub families based on phylogenetic analysis(BLASTP cut-off e-value：1e-10 and identity＞30%).