参考文献

更新于 2026年10月13日 版权声明
参考文献

[1] 胡昭庚.17种药用真菌栽培[M].北京:中国农业出版社,1999.

[2] 李昊,吴白昌,李春兰.虫草人工栽培与深度开发[M].北京:科学技术文献出版社,2003:3-5.

[3] 张平,朱述钧,钱大顺,等.北冬虫夏草功能成分及保健作用分析[J].江苏农业科学,2003(6):105-107.

[4] 葛蓓蕾,江晓路.北虫草的研究现状[J].中国食用菌,1999,18(1):26-27.

[5] 王建芳,杨春清.蛹虫草人工栽培及产品开发研究概况[J].时珍国医国药,2006(2):268.

[6] 文庭池,康冀,雷帮星,等.前体及营养物提高蛹虫草虫草菌素产量的研究[J].食品科学,2010(5):175-179.

[7] 王永敏,祝文兴,安利国,等.优化条件对蛹虫草菌丝体与胞外多糖得率研究[J].济南大学学报,2010(2):148-151.

[8] 李祥玲,胡劲松,陈作红.HPLC测定人工蛹虫草及其培养基中虫草素和腺苷含量[J].湖南师范大学自然科学学报,2010(2):107-111.

[9] Dubois M,Gilies KA,Hamil Ton JK.Colometric method for determination of sugars and related substance[J].Anal Chem,1996,28:350-356.

[10] 蔡友华,范文霞,刘学铭,等.比色法测定巴西虫草菌丝体中虫草酸的含量[J].现代食品科技,2008(1):76-79(https://www.daowen.com)

[11] 张红霞,吴畏,陈伟,等.北冬虫夏草发酵液中虫草素和腺苷含量的HPLC分析[J].上海农业学报,2005(4):53-56

[12] C.-H.Dong,Y.-J.Yao.Nutritional requirements of mycelial growth of Cordyceps sinensis in submerged culture[J].Hourmal of Applied Microbiology,2005,99:483-492.

[13] 邱树毅.生物工艺学[M].北京:化学工业出版社,2009:115-119.

Study on the Active Components Accumulation in Cordceps militaris Throngh Femention Process

Luo Wei1,2  Liu Dong-bo1,2  Wu Zheng-wu1,2  Xia Zhi-lan1,2  Xie Hong-qi1,3

1.College of Horticulture and Landscape,Hunan Agricultural University,Changsha 410128,China

2.State Administration of Traditional Chinese Medicine Sub-health Intervention Technology Laboratory Hunan Agricultural University,Changsha 410128,China

3.Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province,Changsha 410128,China

Abstract:The dynamic variation of the accumulation of biomass,Cordyceps polysaccharides,cordycepic acid and cordycepin were compared through fermentation process.The results showed that 70% of Cordyceps polysaccharide,Cordyceps acid and cordycepin was distributed in the fermentation broth.The optimum fermentation period was 12d by considering these three products.The results of 10 L fermentation tank submerged culture test showed that the biomass reached 24.5 mg/mL,which increased 19.86%compared with shaking flask culture,while the Cordyceps acid,CP,cordycepin content were 7.43mg/mL,2.82mg/m L,90.73μg/mL respectively,which increased 8.3%,13.7% and 15.6%compared with shaking flask culture.

Keywords:Cordyceps militaris,cordycepic acid,cordycepin,Cordyceps polysaccharide

✍AI写作