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Study on bioactive components and antioxidant activity of hull-less pumpkin seed oil

CHEN Tian1，2 　DAISi-hui1 　SHEN Peng-yuan1，2 　HE Xiang-rong1 　ZHONG Qing1 　LIU Dong-bo1，2，3，4

1.College of Horticulture and Landscape，Hunan Agricultural University，Changsha，Hunan 410128，China；

2.State Key Laboratory of Subhealth Intervention Technology，Changsha，Hunan 410128，China；

3.Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization，Changsha，Hunan 410128，China；

4.Hunan Coinnovation Center for Utilization of Botanical Functional Ingredients，Changsha，Hunan 410128，China)

Abstract：The quality indexes of hull-less pumpkin seed oil，which were extracted by supercritical CO2 fluid extraction，were detected according to the national standard.The fatty acid and phytosterol composition were analyzed by gas chromatography-mass spectrometry(GC-MS).Subsequently，the squalene content of hull-less pumpkin seed oil was measured by high performance liquid chromatography(HPLC).Compared with grape seed oil and flaxseed oil，the scavenging activities for DPPH·and ABTS free radical of hull-less pumpkin seed oil were estimated.The results indicate that the hull-less pumpkin seed oil extracted by supercritical CO2 fluid extraction accords with the food industry standard LS/T 3250—2017 pumpkin seed oil.The hull-less pumpkin seed oil mainly contained 4 kinds of unsaturated fatty acid with a total content of 74.73%，which included cis-monounsaturated fatty acids and polyunsaturated fatty acids，with the content of 29.14%，45.54%，respectively.The contents ofβ-sitosterol andΔ-7 phytosterol were 30.75%，10.77%，respectively.Squalene in hull-less pumpkin seed oil was 5 490μg/g，up to 80 times of grape seed oil and 279 times of flaxseed oil.The antioxidant activities of hull-less pumpkin seed oil were positively correlated with its concentration.For the hull-less pumpkin seed oil，its ability to scavenge DPPH·free radical(IC50 16.14 mg/mL)was better than that of grape seed oil and flaxseed oil，and ability to scavenge ABTSfree radical was equivalent to that of grape seed oil and flaxseed oil.Its good antioxidant activity was related to its rich squalene，phytosterol and unsaturated fatty acid.

Keywords：hull-less pumpkin seed oil；unsaturated fatty acid；phytosterol；squalene；antioxidant activity