2.3 Preparation of the Cordycepin Molecularly Impr...
A non-covalent bonding technique was used to prepare the cordycepin molecularly imprinted polymer.Cordycepin was employed as the molecular template.(https://www.daowen.com)
0.1mmol of cordycepin and 0.8mmol of alpha-methylacrylic acid were dissolved in 50.0m L tetrahydrofuran in a three neck flask.The mixture was vigorously mixed in a constant temperature shaker for 24h at 25℃，and 4.0mmol of glycol dimethyl acrylate and 90.0mg of azobisisobutyronitrile were added.The mixture was mixed evenly，sonicated，and degassed with nitrogen for 10min to eliminate oxygen，and sealed for polymerization for 24h at a stirring rate of 250 rpm at 60℃.When the reaction was complete，the polymer was ground with a mortar and pestle，and extracted with methanol for 24h in a Soxhlet device to remove the cordycepin in the polymer and the unreacted material.The product was dried to a constant weight under vacuum at 60℃.The nonimprinted polymer was synthesized and treated with a similar protocol except for the absence of the addition of the cordycepin template during the polymerization phase.