2.4　Static Adsorption Experiments

The 30.0mg of the cordycepin molecularly imprinted polymer or nonimprinted polymer was added into a 10.0mL centrifuge tube，which with 3.0mL of methanol solution contained a certain amount of cordycepin or its structural analogs.The centrifuge tube was shaken at constant temperature of 25℃for 5h，and then the mixture was filtrated after the cordycepin or its structural analogs was adsorbed by the molecularly imprinted polymer or nonimprinted polymer particle.The filtrate was purified with a 0.45μm microfiltration membrane.The concentration of the cordycepin or in the filtration solution was determined by HPLC.The binding capacity of cordycepin，adenosine，and adenine were calculated by the following equation：

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where Q is the binding capacity(μg/g)of the cordycepin molecularly imprinted or the nonimprinted polymer for cordycepin or its structural analogs，C0 is the initial concentrations(μg/L)of cordycepin or its analogs，C1 is the residual concentration(μg/L)of cordycepin or the analogs，V is the solution volume(mL)，and M is the mass(mg)of polymer.