References
Cheng HY，Guo W，Chang MC，Meng JL，Yang J，2011.Study of optimization on liquid fermentation conditions of Cordyceps militaris mycelium.Journal of Shanxi Agricultural University，31(1)：66-72(in Chinese)
Huang M，Zhang S，2010.Research advances of physiological efficacies of cordycepin.Journal of Fungal Research，8(4)：234-244(in Chinese)
Kang JC，Kang C，Wen TC，Qian YX，Lei BX，2011.Pharmacological research progress of cordycepin.Mycosystema，30(2)：191-197(in Chinese)
Kim SK，Kim SW，Lee SC，Kim IW，2008.A pharmaceutical composition comprising cordycepin for the treatment and prevention of obesity.World Intellectual Property Organization.WO2008038973(Al).
Lin QY，Song B，Li TH，2006.Advances in the studies on Cordyceps militaris.Microbiology，33(4)：154-157(in Chinese)
Liu YF，Tang QJ，Gu JJ，Zhang JS，Yang Y，Feng N，Zhou S，Wei DZ，2010.Optimization of submerged fermentation process of Cordyceps militaris to produce a high cordycepin content.Acta Agriculturae Shanghai，26(3)：26-30(in Chinese)
Luan L，Wang GL，Lin RC，2010.Comparative research on HPLC fingerprint of Cordyceps sinensis and its cultured mycelia.Chinese Traditional Patent Medicine，32(6)：893-897(in Chinese)
Luo W，Liu DB，Wu ZW，Xia ZL，Xie HQ，2011.Study on the active components'accumulation in Cordceps militaris throngh femention process.Food and Fermentation Industries，37(9)：108-111(in Chinese)
Mao XB，Eksriwong T，Chauvatcharin S，Zhong JJ，2005.Optimization of carbon source and carbon/nitrogen ratio for cordycepin production by submerged cultivation of medicinal mushroom Cordyceps militaris.Process Biochemistry，40(5)：1667-1672
Mao XB，Zhong JJ，2006.Significant effect of NH4+on cordycepin production by submerged cultivation of medicinal mushroom Cordyceps militaris.Enzyme and Microbial Technology，38(3-4)：343-350
Mina M，Eriko U，Hiromitsu H，Akiniko S，Mikio S，2007.Enhanced production of cordycepin by surface culture using the medicinal mushroom Cordyceps militaris.Emzyme and Microbial technology，40：1199-1205
Mina，Eriko，Akihiko，Mikio，2006.Production of cordycepin by surface culture using the medicinal mushroom Cordyceps militaris.Enzyme and Microbial Technology，39：641-646
Overgaard-hansen K，1964.The inhibition of 5-phosphoribosyl 1-pyrophosphate formation by cordycepin triphosphate in extracts of Ehrlich ascites tumor cells.Biochimica et Biophysica Acta，80：504-507
Wen TC，Lei BX，Kang JC，Li GR，He J，2009.Enhanced production of mycelial and cordycepin by submerged culture using additives in Cordyceps militaris.Food and Fermentation Industries，29(8)：49-52(in Chinese).
Yang T，Dong CH，2011.Cordycepin research and exploitation：progress and problems.Mycosystema，30(2)：180-190(in Chinese)
Ying X，Si LQ，Tu YP，Li G，2009.The comparison of nucleosides in cultured Cordyceps militaris mycelia from different habitats and natural Cordyceps sinensis.Herald of Medicine，28(10)：1354-1356(in Chinese)
Zhang HX，Wu W，Chen W，Gao XH，Tang LS，2005.Analysisof cordycepin and adenosine contents in fermentation supernatant of Cordyceps militaris by HPLC.Acta Agriculturae Shanghai，21(4)：53-56(in Chinese)
Zhu P，Zhu HB，Zhu HX，Zhang L，He HX，Wang Q，Cheng KD，Shi JG，Yang YC，2003.Application of 3＇-deoxyadenosine in the preparation of lipid-lowering drugs.China，200310101650.7［P］.(in Chinese)
程红艳，郭伟，常明昌，孟俊龙，杨杰，2011.蛹虫草菌丝液体发酵培养条件的优化研究.山西农业大学学报，31(1)：66-72(https://www.daowen.com)
黄冕，张松，2010.虫草素生理功效的研究进展.菌物研究，8(4)：234-244.
康冀川，康超，文庭池，钱一鑫，雷帮星，2011.虫草菌素药理学研究进展.菌物学报，30(2)：191-197
林群英，宋斌，李泰辉，2006.蛹虫草研究进展.微生物学通报，33(4)：154-157
刘艳芳，唐庆久，顾俊杰，张劲松，杨焱，冯娜，周帅，魏东芝，2010.北冬虫夏草深层发酵高产虫草素工艺研究.上海农业学报，26(3)：26-30
栾兰，王钢力，林瑞超，2010.冬虫夏草与虫草发酵菌丝体的HPLC指纹图谱比较研究.中成药，32(6)：893-897
罗巍，刘东波，吴郑武，夏志兰，谢红旗，2011.蛹虫草液态发酵中有效成分的动态积累变化.食品与发酵工业，37(9)：108-111
文庭池，雷帮星，康冀川，李光荣，何劲，2009.添加前体促进蛹虫草发酵生产菌丝体和虫草菌素的研究.食品与发酵工业，29(8)：49-52
杨涛，董彩虹，2011.虫草素的研究开发现状与思考.菌物学报，30(2)：180-190
杨昕，斯陆勤，涂轶平，李高，2009.不同产地人工蛹虫草子实体及冬虫夏草中核苷类成分的比较.医药导报，28(10)：1354-1356
张红霞，吴畏，陈伟，高新华，唐乐珊，2005.北冬虫夏草发酵液中虫草素和腺苷含量的HPLC分析.上海农业学报，2005(4)：53-56
朱平，朱海波，朱慧新，张岭，何惠霞，王全，程克棣，石建功，杨永春，2003.3＇-脱氧腺苷在制备降血脂药物中的应用：中国，200310101650.7［P］，2006.05.17
Screening of high-yeild strain and medium optimization for maximum production of cordycepin by Cordyeps militaris
WANG Lei1，2 LUO Wei1，2 HU Xia1，2 LIU Dong-Bo1，2* XIA Zhi-Lan1，2 XIE Hong-Qi1，3*
1.College of Horticulture and Landscape，Hunan Agricultural University，Changsha，Hunan 410128，China；
2.State Administration of Traditional Chinese Medicine Sub-health Intervention Technology Laboratory，Changsha，Hunan 410128，China；
3.Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province，Changsha Hunan 410128，China
Abstract：In order to screen high-yield strain and determine the best harvest time of cordycepin，14 Cordyceps militaris strains was tested by shaking flask fermentation.The effect of 8 different kinds of nutrient additives on the dynamics of cordycepin accumulation were compared.The results showed that C.militaris CM001 strain produced the highest amount of cordycepin.The biomass of C.militaris reached the maximum of 20.44mg/mL at the 10th day while the maximum cordycepin content reached 73.81mg/L at the 13th day.Seventy percent of cordycepin was in the fermentation broth.Additions of adenosine，adenine，alanine，glycine，and L-aspartic acid considerably increased the cordycepin production in the submerged culture，especially the addition of 1g/L adenine which increasing the cordycepin production by 7.09-fold than the control.
Key words：Cordycepsmilitaris，cordycepin，additives，medium optimization