1.3.2　荧光强度分析

从血管腔内表面到血管中心的五个不同位置（区分不同的颜色）收集样本（图1-11）。使用IMAGE-PRO PLUS v.6.0软件分析血管腔不同位置的红色荧光强度。

(https://www.daowen.com)

图1-11　红色荧光强度分析。Flk1：52 h.p.f.的GFP胚胎；在48 h.p.f.注射Dil-LDL的胚胎。内皮细胞为绿色，Dil-LDL为红色。（a）直血管段的红色荧光强度分析。（i，iii，v）来自三个时间点［（i）t=0 s，（iii）t=2.22 s和（v）t=8.88 s］。（ii，iv，vi）荧光强度分析图；小格子标记三个时间点采集的样品。（b）血管分叉区的红色荧光强度分析。（i，iii，v）三个时间点［（i）t=1.674 s， （iii）t=2.511 s和（v）t=13.671 s］。（ii，iv，vi）荧光强度分析图；小格子标记三个时间点采集的样品。［引自：Xie X, et al. In vitro and in vivo investigations on the effects of low-density lipoprotein concentration polarization and haemodynamics on atherosclerotic localization in rabbit and zebrafish. J R Soc Interface, 2013, 10（82）: 20121053.］
Figure 1-11　Red fluorescence intensity analysis. Flk1: GFP embryos at 52 h.p.f. and DiI- LDL embryos at 48 h.p.f. The endothelial cells were green and DiI LDL was red. （a）Red fluorescence intensity analysis of straight vessel segment. Fluorescence intensity analysis diagram from three time points ［（i）t=0 s, （iii）t=2.22 s and （v）t=8.88 s］. （ii, iv, vi）; the samples collected at three time points were marked with a small grid. （b）The red fluorescence intensity of the bifurcation region was analyzed. ［（i）t=1.674 s, （iii）t=2.511 s and （v）t=13.671 s］. The samples collected at three time points were marked with a small grid and fluorescence intensity was analyzed （ii, iv, vi）. ［Adapted from: Xie X, et al. In vitro and in vivo investigations on the effects of low-density lipoprotein concentration polarization and haemodynamics on atherosclerotic localization in rabbit and zebrafish. J R Soc Interface, 2013, 10（82）: 20121053.］